| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367. |
| Cellular Localization | |
| Expression System | |
| Form | Sterile Filtered colorless solution. |
| Formulation | |
| Product Type | |
| Protein Size | |
| Purity | |
| Source | Sf9 insect cells. |
| Species | |
| Storage | |
| Target |
Recombinant Human Proliferating Cell Antigen, S9 is supplied as a recombinant protein for in vitro research use.
Background
Proliferating cell nuclear antigen (PCNA), a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis. Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity (< 10 % of SLE patients). However, their presence alone or in combination with other autoantibodies should alert the clinician to the presence of defined or evolving SLE.
Product format
Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.
Activity & assay information
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Immunological functions: 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera. - Coating concentration: 0.5-1.1 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
What is the purity of Recombinant Human Proliferating Cell Antigen, S9 (Human)?
What buffer / formulation is this protein supplied in?
How should Recombinant Human Proliferating Cell Antigen, S9 (Human) be stored?
What expression system was used to produce this protein?
Is this protein approved for clinical or in vitro diagnostic use?
Can I request a custom size, tag variant, or formulation?
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
