| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | HHG-1;SHH;Sonic Hedgehog Protein |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human Sonic Hedgehog/SHH Protein — also known as HHG-1; SHH; Sonic Hedgehog Protein — Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems; such as limbs and brain. SHH also plays an important role in adult; including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor; and undergoes a series of processi
Research into Human Sonic Hedgehog/SHH Protein supports investigations in tumor biology and oncology signaling, with implications for oncology signaling.
Product Description
E. coli (Prokaryotic)
Produced in E. coli (Prokaryotic), yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
Expression region: Cys24-Gly197.
Protein Specifications
| Molecular Weight (Calculated) | 19.7 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 19 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Expression System | E. coli (Prokaryotic) |
| Endotoxin Level | < 0.01 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refe |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Storage Conditions | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | Q15465 |
| UniProt ID | Q15465 |
| Expression Region | Cys24-Gly197 |
Biological Activity
Activity ValidatedNot validated for activity
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSH033519SpeciesHumanUniProtQ15465
This protein is produced in E. coli (prokaryotic). E. coli expression gives high yield without glycosylation — verify that your assay tolerates non-glycosylated protein.
Purity: > 95% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 0.01 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Not validated for activity. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Please refer to the printed manual for detailed information.. Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
