| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, Speckled 100 kDa, SP100-HMG nuclear autoantigen, FLJ00340, Lysp100b. |
| Expression System | |
| Formulation | |
| Product Type | |
| Protein Size | |
| Purity | |
| Source | Sf9 insect cells. |
| Species | |
| Storage | |
| Target |
Recombinant Human SP100 is supplied as a recombinant protein for in vitro research use.
Background
SP100 protein is Homodimer which takes part in the control of gene expression. SP100 interacts with members of the HP1 family of nonhistone chromosomal protein, such as CBX5 and CBX3 via the PxVxL motif and also interacts with Epstein-Barr virus EBNA-LP. The major isoform Sp100-A, has a calculated MW of 54 kDa, but exhibits aberrant electrophoretic mobilities, with an apparent MW OF 100 kDa.
Product format
Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.
Activity & assay information
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Immunological functions: 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test. - Coating concentration: 0.5-0.9 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
What is the purity of Recombinant Human SP100 (Human)?
What buffer / formulation is this protein supplied in?
How should Recombinant Human SP100 (Human) be stored?
What expression system was used to produce this protein?
Is this protein approved for clinical or in vitro diagnostic use?
Can I request a custom size, tag variant, or formulation?
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
