| Field | Specification |
|---|---|
| Mfr No | |
| Biological Activity | |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Source | Escherichia coli |
| Storage |
Scientific Background
IP-10/CXCL10 also known as CXCL10, is originally identified as an IFN-γ-inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that IP-10 mRNA is also induced by LPS, IL-1β, TNF-α, IL-12 and viruses. Additional cell types that have been shown to express IP-10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. IP-10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human IP-10, Crg-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human IP-10.Recombinant human IP-10/CXCL10 (rhIP-10) produced in E.
Product Description
E. coli ExpressionRecombinant IP-10/CXCL10 Human protein is produced using a validated E. coli expression system and supplied as lyophilized powder for long-term stability. Suitable for use in functional bioassays, ELISA standard curves, receptor binding studies, antibody validation, and related research applications.
Protein Specifications
| Expression System | E. coli |
|---|---|
| Molecular Weight | 8.6 kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% by SDS-PAGE and HPLC analyses. |
| Endotoxin | < 0.2 EU/μg, determined by LAL method. |
| Physical Form | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized after extensive dialysis against 50mM Tris, pH8.0. |
| Reconstitution | Reconstituted in ddH2O at 100 μg/ml. |
| Storage | Lyophilized recombinant human IP-10/CXCL10 (rhIP-10) remains stable up to 6 months at -80°C from dat… |
Functional Activity
ED50 < 0.2μg/ml, measured by a cell proliferation assay of HUVEC cells in the presence of 2.5ng/ml h-VEGF, corresponding to a specific activity of > 5.0× 10ˆ3 IU/mg.
Safety & Handling
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
This protein was produced in E. coli expression system. Expression system selection determines glycosylation profile, folding, and post-translational modifications. For cell-based stimulation assays, verify the expression system matches the glycosylation requirements of your target receptor or pathway.
Reconstituted in ddH2O at 100 μg/ml. Reconstitute in sterile distilled water or PBS at 100 µg/mL as a standard starting concentration. Allow to dissolve at 4°C for 30 minutes without vortexing. Prepare working aliquots in 0.1% BSA carrier protein and store at −80°C.
This protein has been validated for functional bioactivity: ED50 < 0.2μg/ml, measured by a cell proliferation assay of HUVEC cells in the presence of 2.5ng/ml h-VEGF, corresponding to a specific activity of > 5.0× 10ˆ3 IU/mg.. Optimal working concentrations may vary depending on your cell type, assay format, and culture conditions. Titrate the protein in a dose-response experiment to determine the optimal concentration for your system.
Endotoxin level is < 0.2 EU/μg, determined by LAL method. as determined by the LAL method. Low endotoxin is critical for cell-based studies because endotoxin activates NF-κB and TLR4 signalling in immune cells, producing artefactual cytokine induction that can completely mask the true biological activity of the recombinant protein.
Lyophilized recombinant human IP-10/CXCL10 (rhIP-10) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIP-10 should be stable up to 2 weeks at 4°C or up to 3 months a Upon receipt, immediately store at −80°C. Prepare single-use working aliquots to avoid repeated freeze-thaw cycles. Lyophilized protein is stable for 6–12 months at −80°C from the date of receipt.
BioHippo offers flexible sourcing options for qualified research institutions and partners. The following may be available subject to supplier capabilities and order volume.
- Custom quantities: Bulk pricing or non-standard sizes available for high-throughput screening or scale-up projects.
- Custom formulation: Alternative reconstitution buffers or carrier proteins may be accommodated on request.
- Extended QC data: Additional bioactivity assay data, endotoxin reports, or SEC-HPLC purity profiles available on request.
Contact BioHippo customer support to discuss your requirements.
