| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Kex2 protease, also known as a double-alkaline endopeptidase or YSCF protease, is a calcium-dependent serine protease that is responsible for processing the precursors of killer toxin and α-factor within yeast cells. It specifically recognizes and cleaves the peptide bonds at the carboxyl end of dibasic amino acids such as Arg-Arg and Lys-Arg. Unlike trypsin, Kex2 does not recognize and cleave the peptide bonds at the carboxyl end of single basic amino acids, such as arginine or lysine. The activity of Kex2 protease is not inhibited by conventional serine protease inhibitors such as PMSF, TPCK.
YeasenUCF.METM Recombinant Kex2 Protease is expressed in Pichia pastoris, produced under GMP regulations, free of any animal-derived components, and without the risk of viral contamination from animal sources. It has the same enzymatic specificity as the natural Saccharomyces cerevisiae Kex2 enzyme. The optimal pH for activity is pH 9.0, and the stable storage pH is between 5.0 and 6.0.
Features
Strong specificity—Specifically recognizes and cleaves the peptide bonds at the carboxyl end of dibasic amino acids such as Arg-Arg and Lys-Arg.
High purity—Purity≥95%.
Animal free—Recombinantly produced, free from exogenous viral contamination, and no animal-derived materials are used in the production process.
Applications
Processive enzymatic cleavage in peptide drug production.
Enzymatic digestion of proteins and peptide mapping, sequencing, etc.
Specifications
|
Source |
Yeast recombinant expression |
|
Molecular Weight |
Theoretical value 67.0±6.7 kDa |
|
Appearance |
White or off-white powder |
|
Enzyme Concentration |
≥10.0 units/mg pro |
|
Unit Definition |
At 25℃, in a 3 mL reaction system containing 50 mM Tris-HCl and 2 mM CaCl2 at pH 8.0, the amount of enzyme that catalyzes the release of 1 μmoL of 4-nitroaniline from the substrate Boc-QRR-pNA per minute is defined as one unit of enzyme activity. |
|
Quality Assurance |
SDS-PAGE gel detection shows a clear single band of the target protein; no other proteases present, no non-specific cutting. |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
20418ES80 |
20418ES90 |
20418ES91 |
20418ES92 |
|
20418 |
UCF.METM Recombinant Kex2 Protease（Powder） |
1 mg |
10 mg |
100 mg |
1 g |
Shipping and Storage
Lyophilized powder can be stored at 2~8℃ for one year, protected from light and moisture.
Dissolution and Storage Buffer (20 mM NaAc-HAc, pH 5.2, 2 mM Ca2+), after dissolution, store at below -20℃, it is recommended to aliquot for storage to avoid repeated freezing and thawing.
Figure
Figure 1. Yeasen Kex2 protease and carboxypeptidase B efficiently cleave P29-Arg34 GLP-1 (9–37), supporting a tandem enzymatic process with high yield and high purity.
Store this enzyme at 2~8°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Gel Pack and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Processive enzymatic cleavage in peptide drug production. Enzymatic digestion of proteins and peptide mapping, sequencing, etc.. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U/mg) is defined as the amount of protease activity per milligram of enzyme protein under standardized assay conditions (substrate concentration, pH, temperature, and incubation time).
This enzyme is produced as Recombinant (E. coli) and is available in GMP grade for regulated applications. This enzyme is manufactured in an ISO 13485-certified UCF.ME™ facility and meets GMP-grade quality standards. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Most serine proteases (e.g., Proteinase K) are inhibited by PMSF (1 mM) and heat-inactivated at 95°C for 10 min. Proteinase K retains activity across a broad pH range (4–12) and tolerates SDS, urea, and other denaturants, making it particularly effective for nucleic acid extraction from challenging samples including FFPE tissue.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
