| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | AI851531;D930002M15Rik;Nmf350 |
| Endotoxin Level | |
| Formulation | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity.
Alternative names: AI851531;D930002M15Rik;Nmf350
Product Description
Insect Cells (Sf9) ExpressionThis recombinant protein is produced using a validated Insect Cells (Sf9) expression system and supplied as liquid. No reconstitution required. Suitable for biochemical binding assays, ELISA standard curves, antibody validation, protein interaction studies, and related research applications.
Protein Specifications
| UniProt ID | Q9WUA6 |
|---|---|
| Expression System | Insect Cells (Sf9) |
| Expression Region | Ala106-Glu479 |
| Calculated MW | 43.4 kDa |
| Observed MW | 46 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4 |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shelf Life | 12 months |
Functional Activity Data
The specific activity was determined to be 20 nmol/min/mg using synthetic GSK3-derived peptide (R11-SGRARTSSFAEPGGK) as substrate.
Safety & Handling
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
This protein was produced in Insect Cells (Sf9) expression system. The expression system determines the protein post-translational modification profile, folding characteristics, and suitability for specific applications. Mammalian (HEK293) expression is used where native-like glycosylation is required; insect cell and E. coli systems are used for high-yield non-glycosylated production.
This product is supplied as liquid and does not require reconstitution. Centrifuge briefly, then aliquot into single-use working portions in 0.1% BSA carrier protein to minimise freeze-thaw degradation. Avoid repeated freeze-thaw of the bulk stock.
Endotoxin is < 1.0 EU per μg of the protein as determined by the LAL method., determined by the LAL chromogenic method. The <1.0 EU/µg specification ensures suitability for cell culture experiments, primary cell stimulation, and assays measuring immune activation or cytokine secretion, where endotoxin interference with NF-κB signalling is a concern.
This protein meets > 95% as determined by reducing SDS-PAGE purity as assessed by reducing SDS-PAGE. A gel image for each lot is included on the Certificate of Analysis. Contact technical support if purity >98% is required for biophysical measurements such as crystallography, ITC, or single-particle cryo-EM.
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. Shelf life is 12 months from the date of receipt. Prepare small working aliquots to minimise freeze-thaw cycles of the bulk stock. Once thawed, store at 4°C for short-term use (up to 1 week) or refreeze aliquots immediately.
BioHippo offers flexible sourcing and customisation options for qualified research institutions and commercial partners. The following add-ons may be available depending on supplier capabilities and order volume.
- Custom quantities: Bulk orders or non-standard pack sizes may be available for high-throughput or large-scale projects.
- Custom formulation: Alternative buffer compositions or lyophilisation formats may be accommodated on request.
- Biotinylated or labelled variants: Biotinylated, fluorescent, or enzymatic conjugation services may be available for select proteins.
- Extended QC documentation: Endotoxin levels, bioactivity assay data, or additional purity methods can be requested for GMP-adjacent applications.
Contact BioHippo customer support to discuss requirements. Customisation availability and lead times vary by product and supplier.
