| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | AI131712;CAII;Ca2;Car-2;Ltw-5;Lvtw-5 |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Mouse Carbonic Anhydrase II/CA2 Protein — also known as AI131712; CAII; Ca2; Car-2; Ltw-5; Lvtw-5 — The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with
Research into Mouse Carbonic Anhydrase II/CA2 Protein supports investigations in cellular signaling and fundamental cell biology, with implications for cell signaling pathways.
Product Description
E. coli (Prokaryotic)C-His
Produced in E. coli (Prokaryotic), yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
The C-His enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Met 1-Lys 260.
Protein Specifications
| Molecular Weight (Calculated) | 30.4 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 30-33 kDa |
| Purity | > 96% as determined by reducing SDS-PAGE. |
| Affinity Tag | C-His |
| Expression System | E. coli (Prokaryotic) |
| Endotoxin Level | Please contact us for more information. |
| Formulation | Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer infor |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Storage Conditions | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Mouse |
| Accession Number | AAH55291.1 |
| UniProt ID | P00920 |
| Expression Region | Met 1-Lys 260 |
Biological Activity
Activity ValidatedMeasured by its esterase activity. The specific activity is > 100 pmoles/min/μg.
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSM040550SpeciesMouseUniProtP00920
This protein is produced in E. coli (prokaryotic). E. coli expression gives high yield without glycosylation — verify that your assay tolerates non-glycosylated protein.
Purity: > 96% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: Please contact us for more information., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Measured by its esterase activity. The specific activity is > 100 pmoles/min/μg.. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Please refer to the printed manual for detailed information.. Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
