| Field | Specification |
|---|---|
| Accession Number | |
| Alternative Names | AW492086;Cek4;ETK1;End3;Hek;Hek4;Mek4;Tyro4 |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage |
Scientific Background
Mouse EphA3 Protein — also known as AW492086; Cek4; ETK1; End3; Hek; Hek4; Mek4; Tyro4 — EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuro
Research into Mouse EphA3 Protein supports investigations in cellular signaling and fundamental cell biology, with implications for cell signaling pathways.
Product Description
Baculovirus-Insect CellsN-His-GST
Produced in Baculovirus-Insect Cells, yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.
The N-His-GST enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Gly569-Val984.
Protein Specifications
| Molecular Weight (Calculated) | 74.3 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 66 kDa |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Affinity Tag | N-His-GST |
| Expression System | Baculovirus-Insect Cells |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0 |
| Reconstitution | Not Applicable |
| Storage Conditions | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Mouse |
| Accession Number | EDK98238.1 |
| Expression Region | Gly569-Val984 |
Biological Activity
Activity ValidatedKinase activity untested
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSM040288SpeciesMouse
This protein is produced in baculovirus-infected insect cells. Baculovirus/insect expression provides intermediate post-translational modifications between bacterial and mammalian systems.
Purity: > 90% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 1.0 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Kinase activity untested. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Not Applicable. Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
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