| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Galectin-8, Gal-8, LGALS-8, AI326142, D13Ertd524e, 1200015E08Rik. |
| Biological Activity | |
| Concentration | |
| Expression System | |
| Form | Sterile Filtered colorless solution. |
| Formulation | |
| Product Type | |
| Protein Length | |
| Protein Size | |
| Purity | |
| Source | Escherichia Coli. |
| Species | |
| Storage | |
| Target |
Recombinant Mouse Galectin-8 is supplied as a recombinant protein for in vitro research use.
Background
LGALS8 is a prostate-specific antigen that is solely overexpressed in malignant tumors and thus is a supplementary specific identifier of malignancies. LGALS8 is part of the galectin gene family which facilitates both cell-cell and cell matrix interactions in a method parallel to the selectin subgroup of C-type lectins.
Product format
Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.
Activity & assay information
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Assay Conditions: 1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42g, Sodium citric acid 0.8g, Citric acid 0.055g, D-glucose 2.05g in DW100 ml).
2. Centrifuge at 15000rpm for 10 minutes and wash 4 times with PBS.
3. Dilute packed cells in a 0.5mg/ml trypsin-EDTA solution to give 4% red cell suspension.
4. Incubate for 1 hour at 37°C and wash 4 times with PBS.
5. Dilute packed cells in PBS to give a 4% red cell suspension.
6. Load 50µl of 0.5%BSA-in-0.15M-NaCl solution and 25µl of 4%-Red-Cell-in-PBS in U shaped wells.
7. Add 25µl of serial diluted galectin protein in PBS to each well plate (Round bottom 96 well plate).
8. Incubate for 30 minutes at room temperature to observe visible agglutination.
What is the purity of Recombinant Mouse Galectin-8 (Mouse)?
What buffer / formulation is this protein supplied in?
How should Recombinant Mouse Galectin-8 (Mouse) be stored?
What expression system was used to produce this protein?
Is this protein biologically active?
Is this protein approved for clinical or in vitro diagnostic use?
Can I request a custom size, tag variant, or formulation?
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
