| Field | Specification |
|---|---|
| Target | |
| Alternative names | GHBP;GHR/BP;Growth Hormone Receptor |
| UniProt # | |
| Accession number | |
| Species | |
| Applications | |
| Tag | |
| Expression system | |
| Expression region | |
| Molecular weight | |
| Purity | |
| Bioactivity | Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone. The ED50 for this effect is 0.5-2 μg/mL in the presence of 50 ng/mL human growth hormone. |
| Endotoxin level | |
| Formulation | |
| Form | Lyophilized |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Growth hormone receptor, also known as GH receptor and GHR, is a single-pass type I membrane protein which belongs to thetype I cytokine receptor family and type 1 subfamily. GHR contains onefibronectin type-III domain. Growth hormone receptor / GHR is expressed in various tissues with high expression in liver and skeletal muscle. Isoform4of GHR is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform1 expression of GHR in placenta is predominant in chorion and decidua. Isoform4is highly expressed in placental villi. Isoform2of GHR is expressed in lung, stomach and muscle. Growth hormone receptor / GHR is a receptor for pituitary gland growth hormone. It is involved in regulating postnatal body growth. On ligand binding, it couples to the JAK2 / STAT5 pathway. Isoform2of GHR up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. Defects in GHR are a cause of Laron syndrome (LARS) which is a severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may also be a cause of idiopathic short stature autosomal (ISSA) which is defined by a subnormal rate of growth.
Product Description
Expression System: HEK293
Recombinant mouse GHR/GHBP is expressed in HEK293 Cells spanning Met 1-Gln 273, supplied with a C-His-Fc tag. Purity is 85% as determined by reducing SDS-PAGE and the product is provided as a lyophilized preparation.
Protein Specifications
| Expression Host | HEK293 Cells |
|---|---|
| Tag | C-His-Fc |
| Expression Region | Met 1-Gln 273 |
| Calculated MW | 56.8 kDa |
| Observed MW | 70-80 kDa |
| Purity | > 85% as determined by reducing SDS-PAGE. |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Lead Time | 2-3 business days |
| Shelf Life | 12 months |
| Accession Number | NP_034414.2 |
| UniProt # | P16882 |
| Alternative Names | GHBP;GHR/BP;Growth Hormone Receptor |
Functional Activity
Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone. The ED50 for this effect is 0.5-2 μg/mL in the presence of 50 ng/mL human growth hormone.
Safety & Handling
This product is for research use only (RUO) and is not intended for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices.
Related Products
Explore additional recombinant proteins and matched antibodies or ELISA kits for GHR/GHBP research from Elabscience Biotechnology Co Ltd, or contact us for alternative tags and expression formats.
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