| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | ATAR;Atar;HVEM;Herpesvirus entry mediator;HveA;TNF receptor-like molecule;TR2;Tnfrsf14;Tumor necrosis factor receptor superfamily member 14;another TRAF-associated receptor |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage |
Scientific Background
Mouse HVEM/TNFRSF14 Protein — also known as ATAR; Atar; HVEM; Herpesvirus entry mediator; HveA; TNF receptor-like molecule; TR2; Tnfrsf14; Tumor necrosis factor rec — Mouse Protein Tnfrsf14, is a type I transmembrane protein belonging to the TNF receptor superfamily. It is tumor necrosis factor receptor superfamily member 14 and expressed on the surface of T cells during the resting state. Interaction of HVEM with TNF family member LIGHT co-stimulates T cells and promotes inflammation. HVEM also triggers inhibitory signaling cascade in effector T (Teff) cells and regulatory T cells (Tregs) as a ligand of B and T lymphocyte attenuator. Tnfrsf14 is detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. It h
Research into Mouse HVEM/TNFRSF14 Protein supports investigations in cellular signaling and fundamental cell biology, with implications for cell signaling pathways.
Product Description
HEK293 Mammalian CellsC-Fc
Produced in HEK293 Mammalian Cells, preserving native glycosylation patterns and supporting biological activity in cell-based assays.
The C-Fc enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.
Expression region: Gln39-Val207.
Protein Specifications
| Molecular Weight (Calculated) | 455 kDa |
|---|---|
| Molecular Weight (Observed SDS-PAGE) | 50-60 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Affinity Tag | C-Fc |
| Expression System | HEK293 Mammalian Cells |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Storage Conditions | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stab |
| Shelf Life | 12 months |
| Typical Lead Time | 2-3 business days |
| Species | Mouse |
| Accession Number | Q80WM9 |
| Expression Region | Gln39-Val207 |
Biological Activity
Activity ValidatedNot validated for activity
Perform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorElabscience Biotechnology Co LtdCatalog No.PKSM040955SpeciesMouse
This protein is produced in HEK293 mammalian cells. Mammalian expression preserves glycosylation and folding closest to the native protein, supporting biological activity in cell assays.
Purity: > 95% as determined by reducing SDS-PAGE., confirmed by reducing SDS-PAGE. Endotoxin level: < 1.0 EU per μg of the protein as determined by the LAL method., measured by LAL assay — critical for cell-based studies. Identity is verified by peptide mapping or N-terminal sequencing on request.
Yes — biological activity is confirmed per lot. Activity data: Not validated for activity. Run a titration in your own system to establish optimal working concentration before committing all samples.
Reconstitution: Please refer to the printed manual for detailed information.. Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-. Avoid repeated freeze-thaw — each cycle risks partial denaturation.
This protein is validated for ELISA, Functional Assay (In Vitro). It is commonly used as an ELISA standard, binding partner in SPR/BLI, or stimulant in cell-based functional assays. Validate performance in your specific format before use in critical experiments.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
