| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | STE;DER;Fit;Protein T;Protein ST;Lymphocyte antigen;Interleukin-1 receptor-like;suppression of tumorigenicity;IL1RL;IL-1 R;IL-1RL;DER4;Fit-1;IL-1 R4;IL-1RL1;IL-33R;Interleukin-1 receptor-like 1;Lymphocyte antigen 84;Protein ST2;Protein T1;ST2L;ST2V;suppression of tumorigenicity 2;Ly84;St2;Ste2;Il1rl1 |
| Endotoxin Level | |
| Formulation | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
ST2, also called IL-1 R4, is an Interleukin-1 receptor family glycoprotein that plays a role in Th2 immune responses. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. This receptor is very similar to the IL-1 receptor type I and the IL-18 receptor α chain in that ST2 also has three extracellular Ig domains and an intracellular Toll domain. ST2 binds IL-33, enhances inflammatory cytokines by activating nuclear factor-κB (NF-κB) and mitogen activated protein (MAP) kinases. ST2 exists as either a membrane bound form (ST2L) or as a soluble form (sST2).
Alternative names: STE;DER;Fit;Protein T;Protein ST;Lymphocyte antigen;Interleukin-1 receptor-like;suppression of tumorigenicity;IL1RL;IL-1 R;IL-1RL;DER4;Fit-1;IL-1 R4;IL-1RL1;IL-33R;Interleukin-1 receptor-like 1;Lymphocyte antigen 84;Protein ST2;Protein T1;ST2L;ST2V;suppression of tumorigenicity 2;Ly84;St2;Ste2;Il1rl1
Product Description
Mammalian (HEK293) ExpressionThis recombinant protein is produced using a validated Mammalian (HEK293) expression system and supplied as lyophilized powder with C-His affinity tag. Reconstitute as directed on the Certificate of Analysis before use. Suitable for biochemical binding assays, ELISA standard curves, antibody validation, protein interaction studies, and related research applications.
Protein Specifications
| UniProt ID | P14719 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Expression Region | Ser27-Ala337 |
| Affinity Tag | C-His |
| Calculated MW | 36.6 kDa |
| Observed MW | 55-70 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8%… |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -2… |
| Shelf Life | 12 months |
Safety & Handling
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
This protein was produced in Mammalian (HEK293) expression system. The expression system determines the protein post-translational modification profile, folding characteristics, and suitability for specific applications. Mammalian (HEK293) expression is used where native-like glycosylation is required; insect cell and E. coli systems are used for high-yield non-glycosylated production.
This product is supplied as lyophilized powder. Centrifuge the vial briefly before opening, then add the recommended volume of sterile water or the specified buffer and allow to dissolve at 4°C for 30 minutes without vortexing. Prepare single-use working aliquots in 0.1% BSA to minimise freeze-thaw losses of the bulk stock.
Endotoxin is < 1.0 EU per μg of the protein as determined by the LAL method., determined by the LAL chromogenic method. The <1.0 EU/µg specification ensures suitability for cell culture experiments, primary cell stimulation, and assays measuring immune activation or cytokine secretion, where endotoxin interference with NF-κB signalling is a concern.
This protein meets > 95% as determined by reducing SDS-PAGE purity as assessed by reducing SDS-PAGE. A gel image for each lot is included on the Certificate of Analysis. Contact technical support if purity >98% is required for biophysical measurements such as crystallography, ITC, or single-particle cryo-EM.
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. Shelf life is 12 months from the date of receipt. Prepare small working aliquots to minimise freeze-thaw cycles of the bulk stock. Reconstituted lyophilized protein is stable for up to 3 months at −80°C in carrier protein.
BioHippo offers flexible sourcing and customisation options for qualified research institutions and commercial partners. The following add-ons may be available depending on supplier capabilities and order volume.
- Custom quantities: Bulk orders or non-standard pack sizes may be available for high-throughput or large-scale projects.
- Custom formulation: Alternative buffer compositions or lyophilisation formats may be accommodated on request.
- Biotinylated or labelled variants: Biotinylated, fluorescent, or enzymatic conjugation services may be available for select proteins.
- Extended QC documentation: Endotoxin levels, bioactivity assay data, or additional purity methods can be requested for GMP-adjacent applications.
Contact BioHippo customer support to discuss requirements. Customisation availability and lead times vary by product and supplier.
