| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | IFN-Alpha-2;IFNA2;Interferon Alpha-2;Interferon Alpha-A;LeIF A |
| Endotoxin Level | |
| Formulation | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
At least 23 different variants of Interferon-α are known. The individual proteins have molecular masses between 19-26 kD and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxyl-terminal end.
Alternative names: IFN-Alpha-2;IFNA2;Interferon Alpha-2;Interferon Alpha-A;LeIF A
Product Description
E. coli ExpressionThis recombinant protein is produced using a validated E. coli expression system and supplied as lyophilized powder. Reconstitute as directed on the Certificate of Analysis before use. Suitable for biochemical binding assays, ELISA standard curves, antibody validation, protein interaction studies, and related research applications.
Protein Specifications
| UniProt ID | P01573 |
|---|---|
| Expression System | E. coli |
| Expression Region | Cys24-Glu190 |
| Calculated MW | 19.5 kDa |
| Observed MW | 16 kDa |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl, 6% Sucrose, 4… |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -2… |
| Shelf Life | 12 months |
Safety & Handling
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
This protein was produced in E. coli expression system. The expression system determines the protein post-translational modification profile, folding characteristics, and suitability for specific applications. Mammalian (HEK293) expression is used where native-like glycosylation is required; insect cell and E. coli systems are used for high-yield non-glycosylated production.
This product is supplied as lyophilized powder. Centrifuge the vial briefly before opening, then add the recommended volume of sterile water or the specified buffer and allow to dissolve at 4°C for 30 minutes without vortexing. Prepare single-use working aliquots in 0.1% BSA to minimise freeze-thaw losses of the bulk stock.
Endotoxin is < 1.0 EU per μg of the protein as determined by the LAL method., determined by the LAL chromogenic method. The <1.0 EU/µg specification ensures suitability for cell culture experiments, primary cell stimulation, and assays measuring immune activation or cytokine secretion, where endotoxin interference with NF-κB signalling is a concern.
This protein meets > 95% as determined by reducing SDS-PAGE purity as assessed by reducing SDS-PAGE. A gel image for each lot is included on the Certificate of Analysis. Contact technical support if purity >98% is required for biophysical measurements such as crystallography, ITC, or single-particle cryo-EM.
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. Shelf life is 12 months from the date of receipt. Prepare small working aliquots to minimise freeze-thaw cycles of the bulk stock. Reconstituted lyophilized protein is stable for up to 3 months at −80°C in carrier protein.
BioHippo offers flexible sourcing and customisation options for qualified research institutions and commercial partners. The following add-ons may be available depending on supplier capabilities and order volume.
- Custom quantities: Bulk orders or non-standard pack sizes may be available for high-throughput or large-scale projects.
- Custom formulation: Alternative buffer compositions or lyophilisation formats may be accommodated on request.
- Biotinylated or labelled variants: Biotinylated, fluorescent, or enzymatic conjugation services may be available for select proteins.
- Extended QC documentation: Endotoxin levels, bioactivity assay data, or additional purity methods can be requested for GMP-adjacent applications.
Contact BioHippo customer support to discuss requirements. Customisation availability and lead times vary by product and supplier.
