Recombinant Mouse Interleukin 12, Sf9

SKU:BHP11005658
Suppliers
ProSpec-Tany TechnoGene Ltd
ProSpec-Tany TechnoGene Ltd
Details Products
Overview
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RUO recombinant Interleukin 12 (Mouse) protein for mechanistic studies and assay development. Supplied as a traceable protein input (Insect (Sf9/Baculovirus); >95% (SDS-PAGE) purity; sterile-filtered solution; MW 35.7 kDa) to support ELISA.
Target IL12B-IL12A
Species Mouse
Options selector
Catalog no. Size
cyt-1058-2UG 2 ug
cyt-1058-10UG 10 ug
cyt-1058-100UG 100 ug
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size (3) — 2 ug, 10 ug, 100 ug
  • Lead time: options listed as “in stock at manufacturer” typically ship in 5–7 business days; other statuses may take longer.
  • Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No cyt-1058
Alternative Names Interleukin 12 (subunit beta/alpha), IL12b/IL12a, Il-12b/Il-12a, IL-12p40/Il-12p35, Il12p40/Il12p35, p40/p35, Sf9.
Biological Activity The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 for this effect is less or equal to 10ng/ml.
Concentration 0.5 mg/ml
Expression System
  • Insect (Sf9/Baculovirus)
Form Sterile Filtered clear solution.
Formulation IL12 protein solution (0.5mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol.
Product Type
  • Proteins & Peptides
  • Cytokines and Growth Factors
  • Interleukin
Protein Length 335
Protein Size 35.7 kDa
Purity Greater than 95.0% as determined by SDS-PAGE.
Source Sf9, Baculovirus cells.
Species Mouse
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
Target IL12B-IL12A

Recombinant Mouse Interleukin 12, Sf9 is supplied as a recombinant protein for in vitro research use.

Background

interleukin 12 subunit beta/alpha or IL12b/IL12a is a growth factor cytokine which increases the lytic activity of NK/lymphokine-activated killer cells, activated T and NK cells and prompt IFN-gamma production (through resting PBMC). IL12b/IL12a is a crucial part to the process of cellular-immunity and activates the differentiation of Th1 cells originates from the precursor T helper cells. The protein is linked to IL23A and creates the IL-23 interleukin, by that, the autoimmune inflammation is induced and autoimmune inflammatory diseases and tumorigenesis are being affected.

Product format

Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.

What is the purity of Recombinant Mouse Interleukin 12, Sf9 (Mouse)?
Greater than 95.0% as determined by SDS-PAGE. BioHippo includes a Certificate of Analysis (CoA) confirming purity per lot with every order.
What buffer / formulation is this protein supplied in?
Supplied as: IL12 protein solution (0.5mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol. Reconstitute lyophilized material in sterile ultrapure water or the recommended buffer per the datasheet prior to use.
How should Recombinant Mouse Interleukin 12, Sf9 (Mouse) be stored?
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles. Prepare single-use aliquots after reconstitution to avoid repeated freeze–thaw cycles.
What expression system was used to produce this protein?
This recombinant protein was expressed in Insect (Sf9/Baculovirus). The system was selected to achieve high yield, correct folding, and appropriate post-translational modifications.
Is this protein biologically active?
The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 for this effect is less or equal to 10ng/ml. Refer to the product datasheet for recommended assay conditions and controls.
Is this protein approved for clinical or in vitro diagnostic use?
No. Supplied for Research Use Only (RUO) — not intended for therapeutic applications or in vitro diagnostic procedures.
Can I request a custom size, tag variant, or formulation?
Yes. BioHippo can accommodate custom requests including alternative sizes, His/GST/Fc tag variants, bulk quantities, and custom formulations. See the Customization & Add-ons tab or email support@biohippo.com.

Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

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