Recombinant Ovine Prolactin

SKU:BHP11002494
Suppliers
ProSpec-Tany TechnoGene Ltd
ProSpec-Tany TechnoGene Ltd
Details Products
Overview
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RUO recombinant Ovine Prolactin protein for mechanistic studies and assay development. Supplied as a traceable protein input (E. coli; >99% (SDS-PAGE) purity; lyophilized; MW 23 kDa) for reproducible assay inputs.
Target PROLACTIN
Options selector
Catalog no. Size
cyt-240-5UG 5 ug
cyt-240-20UG 20 ug
cyt-240-1MG 1 mg
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size (3) — 5 ug, 20 ug, 1 mg
  • Lead time: options listed as “in stock at manufacturer” typically ship in 5–7 business days; other statuses may take longer.
  • Storage: Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No cyt-240
Alternative Names Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Biological Activity Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Concentration 1 mg/ml
Expression System
  • E. coli
Form Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO 3 .
Product Type
  • Proteins & Peptides
  • Cytokines and Growth Factors
  • Hormone & Adipokine
Protein Length 199
Protein Size 23 kDa
Purity Greater than 99.0% as determined by:<br>(a) Gel filtration analysis.<br>(b) Analysis by SDS-PAGE.
Solubility It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ-cm H 2 O or 0.4% NaHCO 3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Source Escherichia Coli.
Storage Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Target PROLACTIN

Recombinant Ovine Prolactin is supplied as a recombinant protein for in vitro research use.

Background

Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.

Product format

Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.

Quality notes

Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.93 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC DNAman computer analysis program of protein sequences.

What is the purity of Recombinant Ovine Prolactin?
Greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE. BioHippo includes a Certificate of Analysis (CoA) confirming purity per lot with every order.
What buffer / formulation is this protein supplied in?
Supplied as: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO 3 . Reconstitute lyophilized material in sterile ultrapure water or the recommended buffer per the datasheet prior to use.
How should Recombinant Ovine Prolactin be stored?
Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Prepare single-use aliquots after reconstitution to avoid repeated freeze–thaw cycles.
What expression system was used to produce this protein?
This recombinant protein was expressed in E. coli. The system was selected to achieve high yield, correct folding, and appropriate post-translational modifications.
Is this protein biologically active?
Is fully biologically active as evidenced by inducing proliferation of Nb2 cells. Refer to the product datasheet for recommended assay conditions and controls.
Is this protein approved for clinical or in vitro diagnostic use?
No. Supplied for Research Use Only (RUO) — not intended for therapeutic applications or in vitro diagnostic procedures.
Can I request a custom size, tag variant, or formulation?
Yes. BioHippo can accommodate custom requests including alternative sizes, His/GST/Fc tag variants, bulk quantities, and custom formulations. See the Customization & Add-ons tab or email support@biohippo.com.

Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

1. Title :Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets. Publication :Published online before print December 18, 2008, doi: 10.1210/en.2008-1318 Endocrinology April 1, 2009 vol. 150 no. 4 1646-1653 . Link : Prolactin prospec publication 2. Title :ISLET ENDOTHELIAL CELLS AND PANCREATIC ?-CELL PROLIFERATION: STUDIES IN VITRO AND DURING PREGNANCY IN ADULT RATS. Publication :Endocrinology. First published January 26, 2006 as doi:10.1210/en.2005-0997. Link : Prolactin Ovine prospec publication 3. Title :Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets. Publication :Endocrinology. First published ahead of print December 18, 2008 as doi:10.1210/en.2008-1318. Link : Prolactin Protein prospec publication 4. Title: Islet Endothelial Cells and Pancreatic -Cell Proliferation: Studies in Vitro and during Pregnancy in Adult Rats. Publication: Published online before print January26, 2006, doi: 10.1210/en.2005-0997 Endocrinology May 1 , 2006 vol. 147no. 52315-2324. Link: http://endo.endojournals.org/content/147/5/2315.full.pdf+htm l
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