| Field | Specification |
|---|---|
| Mfr No | |
| Cellular Localization | |
| Conjugate | |
| Expression System | |
| Form | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | |
| Product Type | |
| Protein Length | |
| Protein Size | |
| Purity | |
| Shipping | |
| Solubility | It is recommended to reconstitute the lyophilized Streptavidin in sterile 18MΩ-cm H 2 O not less than 0.5mg/ml, which can then be further diluted to other aqueous solutions. |
| Source | Escherichia Coli. |
| Storage | |
| Target |
Recombinant Streptavidin is supplied as a recombinant protein for in vitro research use.
Background
Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
Product format
Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.
Activity & assay information
- Specific Activity: > 17U/mg (one unit binds 1 μg D-biotin).
- Proteolytic Activity: < 10 -3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0).
What is the purity of Recombinant Streptavidin?
What buffer / formulation is this protein supplied in?
How should Recombinant Streptavidin be stored?
What expression system was used to produce this protein?
What are the shipping conditions?
Is this protein approved for clinical or in vitro diagnostic use?
Can I request a custom size, tag variant, or formulation?
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
