| Field | Specification |
|---|---|
| Target | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide of human TRAF6 |
| Conjugate | |
| Purification | |
| Concentration | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. This protein also interacts with the transforming growth factor (TGF) beta receptor complex and is required for Smad-independent activation of the JNK and p38 kinases. This protein has an amino terminal RING domain which is followed by four zinc-finger motifs, a central coiled-coil region and a highly conserved carboxyl terminal domain, known as the TRAF-C domain. Two alternatively spliced transcript variants, encoding an identical protein, have been reported.
Product Overview
This monoclonal antibody (clone R05-2D6), raised in Rabbit, recognizes TRAF6. It is validated for Western Blot.
Validated Applications
| Applications | Western Blot |
|---|---|
| WB | 1:500-1:1000 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
A synthetic peptide of human TRAF6
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Monoclonal |
| Clone | R05-2D6 |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Rat |
| Immunogen | A synthetic peptide of human TRAF6 |
| Purification | Affinity Purified |
| Concentration | 300 μg/mL |
| Storage Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein |
| Calculated MW | 60kDa |
| Observed MW | 60kDa |
| UniProt # | Q9Y4K3 |
| Lead Time | 2-3 business days |
| Validity | 12 months |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice packs |
| Target Synonyms | E3 ubiquitin-protein ligase TRAF6;Interleukin 1 signal transducer;Interleukin-1 signal transducer;MGC 3310;MGC:3310;MGC3310;OTTHUMP00000232772;OTTHUMP00000232773;RING finger protein 85;RNF 85;RNF85;TNF receptor associated factor 6;TNF receptor-associated factor 6;TNF receptor-associated factor 6;E3 ubiquitin protein ligase;TRAF 6;Traf6;TRAF6 |
Reactivity Notes
Validated reactivity: Human, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for TRAF6 research from Elabscience Biotechnology Co Ltd, or contact us for alternative formats.
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