| Field | Specification |
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| Target | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant protein of human TRIM21 |
| Conjugate | |
| Purification | |
| Concentration | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
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Scientific Background
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus.
Product Overview
This monoclonal antibody (clone R01-1E8), raised in Rabbit, recognizes TRIM21. It is validated for Western Blot.
Validated Applications
| Applications | Western Blot |
|---|---|
| WB | 1:500-1:1000 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Recombinant protein of human TRIM21
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Monoclonal |
| Clone | R01-1E8 |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human TRIM21 |
| Purification | Affinity Purified |
| Concentration | 300 μg/mL |
| Storage Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein |
| Calculated MW | 54kDa |
| Observed MW | 50kDa |
| UniProt # | P19474 |
| Lead Time | 2-3 business days |
| Validity | 12 months |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice packs |
| Target Synonyms | 52 kDa ribonucleoprotein autoantigen Ro/SS-A;52 kDa Ro protein;52kD Ro/SSA autoantigen;Autoantigen Ro/SSA;52-KD;E3 ubiquitin-protein ligase TRIM21;RING finger protein 81;RNF81;Ro 52;Ro(SS-A);Ro52;RO52;Sicca syndrome antigen A;Sjoegren syndrome type A antigen;Sjogren syndrome antigen A1;Sjogren syndrome type A antigen;SS-A;SSA;SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen S |
Reactivity Notes
Validated reactivity: Human. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for TRIM21 research from Elabscience Biotechnology Co Ltd, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
