| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Trypsin is a serine protease secreted by the pancreas, which recognizes the sites of lysine or arginine in proteins and cuts at the carboxyl end of the lysine or arginine residues. This product is a recombinant porcine trypsin expressed by Pichia pastoris, produced under GMP regulations, free of any animal-derived components, and free from animal virus contamination (such as swine influenza virus, porcine parvovirus, etc.). It naturally lacks chymotrypsin activity and has been filtered to remove bacteria. The activity of this product is inhibited by serine protease inhibitors such as PMSF and TLCK, and metal ion chelating agents such as EDTA.
Yeasen Recombinant Trypsin activity is based on the internationally accepted activity (USP) and is superior to imported similar products. The HPLC content of β-Trypsin can reach over 80%, with high storage stability and a proteomics enzymatic digestion miss-cut rate that is 50% lower than imported products.
Features
1.High activity，good stability.
2.Free of any protease inhibitors，such as PMSF.
Applications
Protein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis.
Specifications
|
Source |
Yeast recombinant expression |
|
Purity |
＞95% |
|
Concentration |
2.0 mg/mL |
|
Specific activity |
＞6800 USP Unit/mg |
|
β-Trypsin |
＞80% |
|
α-Trypsin |
＜20% |
|
Storage Buffer |
1 mM HCL |
|
Unit Definition |
At 25°C, pH 7.6, in a reaction system of 3.2 mL (1 cm light path), the increase in absorbance at 253 nm by 0.003 per minute during the enzymatic digestion of BAEE is defined as one USP unit. |
Glycerol Content |
Contains Glycerol |
Storage
The product can be stored at -25 ~ -15℃ for two years.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Protein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis.. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U/mg) is defined as the amount of protease activity per milligram of enzyme protein under standardized assay conditions (substrate concentration, pH, temperature, and incubation time).
This enzyme is produced as Recombinant (E. coli) and is available in GMP grade for regulated applications. This enzyme is manufactured in an ISO 13485-certified UCF.ME™ facility and meets GMP-grade quality standards. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Most serine proteases (e.g., Proteinase K) are inhibited by PMSF (1 mM) and heat-inactivated at 95°C for 10 min. Proteinase K retains activity across a broad pH range (4–12) and tolerates SDS, urea, and other denaturants, making it particularly effective for nucleic acid extraction from challenging samples including FFPE tissue.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
