Recombinant WEEV E2 GP (E3E2) - (mammalian)

What it means: The expression system is the host platform used to produce this recombinant protein. This antigen is expressed in HEK-293T cells, which shapes its structure and modifications.

Why it matters:

• Expression in HEK-293T cells determines post-translational modifications such as glycosylation and disulfide-bond formation.
• Those modifications govern how closely the recombinant antigen mimics the native protein in folding, stability and immunoreactivity.
• The system also influences endotoxin level and the kinds of downstream assays the protein is best suited to.

How to interpret here: This protein is produced in HEK-293T cells, so expect the folding and modification profile typical of that platform when planning conformational or glycan-dependent work.

Tip: For assays that depend on native glycosylation, confirm the expression system matches your experimental requirements.

What it means: Form describes the physical state in which the product is supplied. This protein ships as a liquid preparation, which sets how you handle it on receipt.

Why it matters:

• The liquid form determines the handling, aliquoting and any reconstitution steps required before use.
• It dictates the cold-chain shipping and short-term storage arrangements you should prepare for.
• Knowing the form in advance lets you plan single-use aliquots and avoid repeated freeze-thaw damage.

How to interpret here: This product is supplied in liquid form; prepare your storage, aliquoting and handling workflow accordingly.

Tip: Dispense into small single-use aliquots immediately on receipt to preserve activity.

What it means: The target pathogen indicates the infectious agent this recombinant antigen is derived from — Western Equine Encephalitis Virus.

Why it matters:

• Correct Western Equine Encephalitis Virus antigen selection is fundamental to serology, assay development and host-pathogen research.
• The strain and lineage of the Western Equine Encephalitis Virus antigen can change which antibodies and sera recognise it.
• Antigen provenance should be documented so results can be compared across studies and reagent lots.

How to interpret here: This antigen represents Western Equine Encephalitis Virus; match it to the antibodies, sera or assays in your study.

Tip: Select an antigen strain that corresponds to the antibody panel or serology you intend to run.

What it means: Concentration states the amount of protein supplied per unit volume. This product is provided at Batch dependent within range: 100 μg at 0.3-3 mg/mL.

Why it matters:

• Knowing the concentration lets you calculate accurate dilutions, coating amounts and assay inputs.
• A batch-dependent value means the precise figure varies by lot and must be read from the lot-specific certificate or datasheet.
• Accurate concentration data underpins reproducible quantitative work and inter-lot comparison.

How to interpret here: Use the stated concentration (Batch dependent within range: 100 μg at 0.3-3 mg/mL) for planning, and confirm the exact lot value before any quantitative experiment.

Tip: Record the lot-specific concentration in your assay notes for traceability.

What it means: Storage gives the manufacturer-recommended conditions for maintaining product stability and activity: Store at -20°C for 2-3 weeks, -80°C for long term.

Why it matters:

• Following the storage guidance preserves antigen integrity, folding and binding activity over time.
• Improper temperature or repeated freeze-thaw cycling is a common, avoidable cause of lost activity and inconsistent results.
• Correct storage also protects the value of the reagent across multiple experiments and users.

How to interpret here: Store this protein exactly as stated (Store at -20°C for 2-3 weeks, -80°C for long term) from the moment it arrives.

Tip: Aliquot before freezing so each experiment uses a fresh tube and freeze-thaw cycles are minimised.