Recombinant ZAP70 Antibody

Overview

Zeta-associated protein-70 (ZAP-70) is a member of the Syk family of tyrosine kinases, a group of proteins that attach to the zeta chain components of T-cell receptors to signal downstream events involved in the regulation of cell function, proliferation, and death. Research suggests that the ZAP-70 protein may also play an important role in natural killer (NK) cell activation and early B-cell development; however, it is not expressed in most normal mature B-cells. Expression of ZAP-70 has been reported in various lymphomas, including mantle cell lymphoma, small lymphocytic lymphoma and marginal zone lymphoma. During thymocyte development, ZAP-70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP-70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

This anti-ZAP70 antibody is supplied as Purified (Rabbit, Recombinant Rabbit Monoclonal, clone ZAP70/4040R, Rabbit IgG, Unconjugated) and is designed to support common target-detection workflows after the on-page specifications.

Key elements and design rationale

• Target: ZAP70
• Format: Purified
• Localization: Cell surface, cytoplasm
• Species reactivity: Human
• Applications (listed): ELISA, IHC-P
• Conjugate: Unconjugated
• Clone and antibody class: Recombinant Rabbit Monoclonal, clone ZAP70/4040R, Rabbit IgG

Because antibody performance can depend on epitope context, sample preparation, and biological state, interpret signals using appropriate controls and orthogonal evidence when possible.

Biological background

ZAP70 is referenced in public gene/protein resources (e.g., UniProt and NCBI Gene), which provide curated names/synonyms, protein features, and pathway context. When designing assays, consider potential isoforms, post-translational modifications, and cell-type specific expression that may influence observed signal.

Research relevance and current trends

• Profiling ZAP70 expression across model systems, perturbations, and time points to support mechanistic hypotheses.
• Combining antibody-based detection with multi-omics or imaging readouts to link ZAP70 signal with phenotype.
• Using well-matched controls (isotype controls, genetic perturbations, or independent reagents) to strengthen interpretation of target-associated signal.

Common research applications

• ELISA
• IHC-P

Use the listed applications as a starting point and tailor experimental design to your sample type and readout requirements.

Notes for experimental interpretation

• Specificity considerations: closely related family members, isoforms, or PTMs can affect apparent specificity; confirm with independent approaches when critical.
• Controls: include negative controls and, when feasible, genetic or pharmacologic perturbations to support target attribution in your system.
• Species and sample context: differences in sequence, expression, fixation, or extraction conditions can change signal behavior across models.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.