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RenCa (Renal Carcinoma) cells are a murine renal adenocarcinoma cell line. They are derived from a tumor spontaneously developed in the kidney of a BALB/c mouse, a common inbred strain used in research. RenCa cells are used extensively to study renal cancer biology, tumor immunology, and cancer therapy, including the efficacy of immunotherapeutic agents. The cells are known for their aggressive tumor formation when implanted in syngeneic mice, making them a valuable model for in vivo experiments that aim to mimic cancer progression and metastasis in a controlled laboratory environment.
RenCa cells are characterized by a high mitotic index and are able to grow in an anchorage-independent manner, forming colonies in soft agar, which is a hallmark of oncogenic transformation. They display a fibroblast-like morphology and due to their origin in a BALB/c mouse, RenCa cells are particularly useful for research using immunocompetent mice, facilitating studies on the interaction between cancer cells and the immune system. This cell line has been utilized in numerous studies investigating the role of specific immune cells and molecules in tumor growth suppression and the potential for therapeutic intervention.
In addition to their use in immunotherapy research, RenCa cells have also served as a tool in the study of cancer metastasis mechanisms, particularly in the context of the renal system. They have been employed to assess the impact of various genes and proteins on tumor invasiveness and metastatic potential, offering insights into the pathways that might be targeted to inhibit cancer spread in renal carcinoma. These features make RenCa a crucial model in both fundamental and translational cancer research.
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- Tumorigenic: Yes, in syngeneic mice
- Virus susceptibility: MAP testing negative (Sendai, Ektromelie, Polyoma, K-Virus, Kilham, LCM, M.pulmonis, MVM, Theiler`s GD VII, toolan`s H-1, MHV, RCV/SDA, M-Adenovirus)
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- doublingTime: 47 hours
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 2 x 104 cells/cm2
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: Fast. Viability 93%. Allow cells to recover from the freezing process for 24 to 48 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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