| Field | Specification |
|---|---|
| Target | |
| Alternative names | RFWD3; RNF201; E3 ubiquitin-protein ligase RFWD3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423 |
| Conjugate | |
| Concentration | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 3 WD repeats.,
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes RFWD3. It is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA.
Validated Applications
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
|---|---|
| Western Blot | 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423 |
| Concentration | 1 mg/mL |
| Observed Band | 85kD |
| Subcellular Location | Nucleus . Nucleus, PML body . Cytoplasm . In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies (PubMed:21558276). In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair (PubMed:21504906, PubMed:28575657). This recruitment depends upon RPA2 (PubMed:21504906). . |
| Swiss-Prot | Q6PCD5 |
| Gene ID | 55159 |
| Storage | -20°C/1 year |
Reactivity Notes
Validated reactivity: Human, Rat, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for RFWD3 research from ELK Biotechnology, or contact us for alternative formats.
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