| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Puromycin + Blasticidin |
| Shipping | |
| Species |
Background
RIG-I (retinoic acid-inducible gene I, encoded by DDX58) is a cytosolic pattern recognition receptor that detects viral RNA, particularly short double-stranded and 5'-triphosphate RNA, during infection. Upon recognizing viral RNA, RIG-I signals through the adaptor MAVS to activate transcription factors that induce type I interferons. Secreted interferons then act through the JAK/STAT pathway to assemble the ISGF3 complex, which binds interferon-stimulated response elements (ISRE) in the promoters of antiviral genes. The ISRE element therefore reports the output of RIG-I-initiated innate immune signaling, making RIG-I a key target in antiviral immunity and interferon biology research.
Product Description & Applications
The RIG-1/ISRE Reporter Lentivirus Set is an immunotherapy reporter system supplied as two vials. A receptor lentivirus constitutively expresses human RIG-I with antibiotic selection, while a reporter lentivirus carries tandem ISRE response elements driving a dual reporter, secreted Gaussia luciferase together with eGFP or mCherry. Sequential transduction and selection generates a dual-stable cell line that produces a quantitative readout when RIG-I-initiated signaling activates ISRE-dependent transcription.
Secreted Gaussia luciferase accumulates in conditioned media, allowing kinetic sampling without cell lysis, while fluorescence supports microscopy and flow cytometry. The VSV-G-pseudotyped particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, including primary and thawed cultures. The system supports antiviral innate immunity assays and study of interferon signaling.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human RIG-1 under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (Gaussia Luciferase + eGFP, Gaussia Luciferase + mCherry). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
