| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CARD 3; CARD carrying kinase; CARD containing ICE associated kinase; CARD containing IL 1 beta ICE kinase; CARD containing IL1 beta ICE kinase; CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase; CARD containing interleukin 1 beta converting enzyme associated kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARD3; CARDIAK; CCK; CLARP kinase; GIG 30; GIG30; Growth inhibiting gene 30; Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase; Receptor interacting protein 2; Receptor interacting serine threonine kinase 2; Receptor interacting serine/threonine protein kinase 2; Receptor-interacting protein 2; Receptor-interacting serine/threonine-protein kinase 2; RICK; RIP 2; RIP like interacting CLARP kinase; RIP-2; RIP-like-interacting CLARP kinase; RIPK 2; Ripk2; RIPK2_HUMAN; TNFRSF; Tyrosine-protein kinase RIPK2; UNQ277/PRO314/PRO34092 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human Receptor-interacting serine/threonine-protein kinase 2 protein (1-540AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-RIPK2 antibody raised in Rabbit, with confirmed utility in ELISA, WB, IHC, IF. Designed to detect RIPK2 in Human, Rat, supporting researchers in cell biology contexts.
Key elements and design rationale
- Immunogen: Recombinant Human Receptor-interacting serine/threonine-protein kinase 2 protein (1-540AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
- Host (Rabbit): Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
- Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
- Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A/G compatibility.
- Purification (Protein G purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
Biological background
RIPK2 (also known as CARD 3, CARD carrying kinase, CARD containing ICE associated kinase, CARD containing IL 1 beta ICE kinase, CARD containing IL1 beta ICE kinase, CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase, CARD containing interleukin 1 beta converting enzyme associated kinase, CARD-containing IL-1 beta ICE-kinase, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD3, CARDIAK, CCK, CLARP kinase, GIG 30, GIG30, Growth inhibiting gene 30, Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase, Receptor interacting protein 2, Receptor interacting serine threonine kinase 2, Receptor interacting serine/threonine protein kinase 2, Receptor-interacting protein 2, Receptor-interacting serine/threonine-protein kinase 2, RICK, RIP 2, RIP like interacting CLARP kinase, RIP-2, RIP-like-interacting CLARP kinase, RIPK 2, Ripk2, RIPK2_HUMAN, TNFRSF, Tyrosine-protein kinase RIPK2, UNQ277/PRO314/PRO34092) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of RIPK2 biology relevant to cell biology.
Common research applications
- ELISA: Sandwich or indirect ELISA quantifies soluble/secreted target in biological fluids or cell culture supernatants.
- WB: Western blot confirms target MW and detects isoforms/fragments under denaturing conditions.
- IHC: IHC visualizes target expression in FFPE or frozen sections; optimize antigen retrieval and secondary detection per tissue type.
- IF: IF localizes target at subcellular resolution in fixed cells/sections; fixation and permeabilization conditions affect epitope accessibility.
Notes for experimental interpretation
- Isotype controls: Include IgG from Rabbit at equivalent concentration to assess non-specific background.
- Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
- Species reactivity: Confirmed for Human, Rat; extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
RIPK2 is a protein target in Human, Rat biology. This polyclonal antibody raised in Rabbit detects RIPK2 and is validated for ELISA, WB, IHC, IF applications.
This antibody is reported reactive against Human, Rat. Immunogen derived from Human. Cross-reactivity with unlisted species should not be assumed — contact us or consult the datasheet for homology data.
Supplier reports performance in ELISA, WB, IHC, IF. Dilution optimization, blocking, and appropriate controls are required per format. Contact BioHippo for recommended working concentrations.
This non-conjugated (IgG) antibody requires a secondary anti-rabbit IgG conjugated to HRP, AP, fluorophore, or biotin. Match the secondary to the host (Rabbit) and isotype (IgG).
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — lysate/cell line expressing RIPK2; (3) Negative control — siRNA KD or KO sample to confirm specificity; (4) Dilution linearity — verify proportional signal decrease for quantitative applications.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
