| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C29H25FN4O4 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
RIPK3-IN-1 is a RIPK3 type II DFG-out inhibitor with an IC50 of 9.1 nM. It also inhibits RIPK1 and RIPK2, with IC50 values of 5.5 μM and >10 μM, respectively, and acts as a c-Met kinase inhibitor with an IC50 of 1.1 μM[1]. It is supplied as a light yellow to yellow solid (C29H25FN4O4, MW 512.53) at 99.30% purity.
Physical & Chemical Properties
| CAS Number | 2361139-70-8 |
|---|---|
| Molecular Formula | C29H25FN4O4 |
| Molecular Weight | 512.53 g/mol |
| Purity | 99.30% |
| Appearance | Solid |
| Color | Light yellow to yellow |
| SMILES | O=C(C1=CC=CN(C2=CC=C(F)C=C2)C1=O)NC3=CC=C(OC4=CC(NC(C5CC5)=O)=NC=C4)C(C)=C3C |
| Target | RIPK3 |
| Signaling Pathway | Apoptosis |
| Solubility | In Vitro: DMSO: 5 mg/mL (9.76 mM; Requires sonication and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 2 years; -20°C, 1 year. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
|
RIPK3 9.1 nM (IC50) |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 5 mg/mL (9.76 mM) | requires sonication and warming and heat to 80°C; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 2 years) or -20°C (up to 1 year); avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
ABL, BRAF/V599E, MAP4K3, and SRC are also inhibited by RIPK3-IN-1, with IC50s of 0.37, 0.15, 0.012, and 0.075 μM, respectively[1]. Necroptosis, a programmed form of cell death, has been linked to many diseases, among them ischemia reperfusion injury, neurodegenerative disorders, pancreatic cancer, and autoimmune conditions such as inflammatory bowel disease (IBD). Stimulation of death receptors (for example, the TNF receptor family) can trigger signaling that starts necroptotic cell death. The process involves a necrosome, a cytosolic complex containing receptor interacting protein kinases 1 and 3 (RIPK1, RIPK3)[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Loss of Ripk3 attenuated neutrophil accumulation in a lipopolysaccharide-induced zebrafish inflammatory model. Cell Death Discov 2022 Feb 26;8(1):88. PMID: 35220408
