| Field | Specification |
|---|---|
| Applications | |
| Source | Recombinant (E. coli) |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
RNase Inhibitor is a recombinant RNase inhibitor expressed in soluble form in Escherichia coli. It performs equivalently to the ribonuclease inhibitor purified from human placenta. It is a protein of approximately 51 kDa with an isoelectric point (pI) of 4.7. RNase Inhibitor can specifically bind RNase A, B, and C with a non-covalent bond to form a 1:1 complex to inactivate RNase, and has a broad spectrum of RNase inhibitory activity. The inhibitor is active in buffers of 0–0.5 M NaCl, pH 5–8, and has the highest activity at pH 7.8. It protects the integrity of mRNA and improves the efficiency of transcription and translation, while avoiding the possible effects of using organic compound inhibitors. The inhibitor is compatible with commonly used reverse transcriptases and DNA polymerases in RT-PCR and RT-qPCR. Unlike the human placental inhibitor, the recombinant protein lacks two cysteine residues and is therefore more resistant to oxidation, making it better suited to reactions that are sensitive to high DTT, such as qPCR.
The inhibitor is supplied as a single-component solution at 40 U/µL in 20 mM HEPES-KOH (pH 7.5), 50 mM KCl, 5 mM DTT and 50% glycerol. One activity unit is the amount of enzyme that inhibits 50% of the activity of 5 ng of RNase A. SDS-PAGE shows a single band at a molecular weight of 50 kDa.
Specifications
| Concentration | 40 U/µL |
|---|---|
| Unit definition | Amount of enzyme that inhibits 50% of the activity of 5 ng of RNase A |
| Source | Recombinant (E. coli) |
| Molecular weight | ~51 kDa (SDS-PAGE shows a single band at ~50 kDa) |
| Isoelectric point | pI 4.7 |
| Specificity | Binds RNase A, B and C non-covalently to form a 1:1 complex |
| Active range | pH 5–8 in buffers of 0–0.5 M NaCl |
| Optimal pH | 7.0–8.0; highest activity at pH 7.8 |
| Composition | 20 mM HEPES-KOH (pH 7.5), 50 mM KCl, 5 mM DTT, 50% glycerol |
Applications
- First-strand cDNA synthesis
- Isolation of polysomes
- In vitro translation
- In vitro cell-free system transcription
- In vitro transcription with SP6 or T7 RNA polymerase
Storage & Handling
Stored at -20°C with a shelf life of one year.
Usage Notes
- Foaming or strong agitation such as vortexing can cause deactivation.
- Use the inhibitor within its active range of pH 5–8 and 0–0.5 M NaCl.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
