| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Storage |
RNase R is an E. coli exoribonuclease that exhibits 3'-to-5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double-stranded RNA with short 3’ overhangs (less than seven nucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing. This enables researchers to probe the landscape of splicing events with greater depth.
| Product Component | Quantity |
|---|---|
| RNase R (10 U/µl) | 50 µl |
| 10X RNase R Reaction Buffer | 1.0 ml |
| Specification | Value |
|---|---|
| Enzyme Type | RNase |
| Format | Enzyme Only |
| 3'→5' Proofreading | Yes |
| Storage Conditions | Store all components at -20 °C. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. All components are stable for 1 year from the date of shipping when stored and handled properly. |
RNase R is a highly processive 3′→5′ exoribonuclease that degrades linear single-stranded and double-stranded RNA but is unable to degrade circular RNA. This selectivity makes it the preferred enzyme for enriching circular RNA from total RNA preparations.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
References
- De Tomi E; Orlandi E; Belpinati F et al.. New Axes of Interaction in Circ_0079593/miR-516b-5p Network in Melanoma Metastasis Cell Lines. Genes. 2024;15. DOI
- Hu Q; Zhao H; Zhou K et al.. Scarless circular mRNA-based CAR-T cell therapy elicits superior antitumor efficacy. Signal transduction and targeted therapy. 2025;10:411. DOI
- Wesselhoeft RA; Kowalski PS; Parker-Hale FC et al.. RNA Circularization Diminishes Immunogenicity and Can Extend Translation Duration In Vivo. Molecular cell. 2019;74:508-520.e4. DOI
