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Pair one of abm's best-selling products with our high performance RNaseOFF Ribonuclease Inhibitor!
RNase R (Cat. No. E049) is an E. coli exoribonuclease that exhibits 3'-to-5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double-stranded RNA with short 3’ overhangs (less than seven nucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing. This enables researchers to probe the landscape of splicing events with greater depth. To further the protection and enhancement of circRNAs, this bundle includes our high performance RNaseOFF Ribonuclease Inhibitor (Cat. No. G138S) for one special price!
This bundle includes:
- RNase R (500 U,10 U/µl)
- 10X RNase R Reaction Buffer
- RNaseOFF Ribonuclease Inhibitor (800 U, 40 U/µl)
| Specification | Value |
|---|---|
| Enzyme Type | RNase Inhibitor |
| Format | Kit |
| 3'→5' Proofreading | Yes |
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This ribonuclease inhibitor protects against RNase A, B, and C. It binds RNase with sub-picomolar affinity (Ki ≈10⁻¹⁴ M) and is active across pH 5.5–8.5. Include DTT or β-mercaptoethanol (1–5 mM) to maintain the inhibitor in its reduced, active form.
Recommended usage is 1–4 U/µL of reaction volume. For co-immunoprecipitation of RNA-binding proteins or sensitive single-cell applications, 4–8 U/µL is advised to ensure complete RNase suppression.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
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