RNase R with RNaseOFF Bundle

What it means: Bundle Contents lists what ships in the box. This pack combines RNase R with RNaseOFF Ribonuclease Inhibitor — one enzyme that removes linear RNA and one that blocks contaminating ribonucleases.

Why it matters:

• The two enzymes do opposite things on purpose: RNase R digests the linear RNA you want gone, RNaseOFF protects what is left from RNases you did not intend.
• Buying them together removes the risk of running the enrichment without protection, which is the commonest way circRNA yield is lost.
• The bundle is priced as a single item rather than two separate lines.

How to interpret here: The pack supplies RNase R (500 U, 10 U/µl), 10X RNase R Reaction Buffer, and RNaseOFF Ribonuclease Inhibitor (800 U, 40 U/µl). Both components can also be bought separately as Cat. Nos. E049 and G138.

Tip: RNaseOFF does not inhibit RNase R's intended activity — it targets contaminating RNase A-family enzymes.

What it means: Primary Use Case names the workflow the bundle is assembled for — enriching circular RNA from a total RNA preparation ahead of sequencing.

Why it matters:

• circRNAs are vastly outnumbered by linear transcripts, so without depletion they are nearly invisible in RNA-seq.
• Depletion and protection are two separate failure points; addressing only one still loses the signal.
• Resistance to RNase R digestion is itself part of the evidence that a transcript is circular, so the treatment is analytical as well as preparative.

How to interpret here: Treat total RNA with RNase R in the supplied buffer, with RNaseOFF present to guard the surviving RNA, then proceed to library preparation. Compare treated and untreated libraries rather than treated alone.

Tip: Validate individual circRNA candidates with divergent primers — duplexes with short 3′ overhangs also survive digestion.

What it means: RNaseOFF Included states how much inhibitor comes in the bundle — 800 U at 40 U/µl, the same formulation sold on its own as Cat. No. G138S.

Why it matters:

• Knowing the unit count lets you plan how many enrichment reactions the pack covers rather than dosing by volume.
• This inhibitor resists oxidation and stays active below 1 mM DTT, so it works in low-reducing-agent protocols where classic inhibitors fail.
• It blocks RNase A, B and C without inhibiting downstream polymerase activity, so it can stay in the sample through cDNA synthesis.

How to interpret here: 800 U is sized to match the 500 U of RNase R in the same box, so the two components run out at roughly the same rate under normal use.

Tip: Keep the inhibitor on ice and avoid repeated freeze-thaw — oxidation resistance is not the same as indefinite stability.