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Same performance as competing brands, better pricing!
RNaseOFF Ribonuclease Inhibitor is an advanced enzyme designed to provide superior protection for RNA in sensitive experiments. Specifically engineered to inhibit common ribonucleases (RNases), including RNase A, B, and C, it offers high-affinity protection against potential RNase contamination. This makes RNaseOFF an invaluable additive in PCR and RT-PCR applications, ensuring that RNA remains intact without inhibiting polymerase activity. Unlike traditional human RNase inhibitors, RNaseOFF is more resistant to oxidation, offering enhanced stability even under challenging conditions. It is particularly stable at low concentrations of DTT (< 1 mM), making it the optimal choice for experiments requiring minimal reducing agents. With its ability to safeguard RNA while maintaining polymerase efficiency, RNaseOFF is the ideal solution for achieving reliable, high-quality results in your research.
- High stability at 70ºC
- High resistance to oxidation
- No downstream inhibition
- 60% more affordable compared to competing brands at <$0.02/U
- Larger size available (Cat. No. G591)
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Customer Testimonial "My lab has been using RiboLock RNase inhibitor from Fermentas for several years. We have tried Sample-G138 and cross-compared efficiency of cDNA synthesis and qPCR after immunoprecipitation of RNA-binding proteins using HeLa cell lysates supplemented with each RNase inhibitor. We do not see gross difference of Ct values from cDNA samples synthesized from immunoprecipitates samples." - Je-Hyun Yoon, The Medical University of South Carolina |
| Specification | Value |
|---|---|
| Enzyme Type | RNase Inhibitor |
| Format | Enzyme Only |
| RNase Specificity | RNase A, B, C |
| Storage Conditions | Store at -20°C. |
This ribonuclease inhibitor protects against RNase A, B, C. It binds RNase with sub-picomolar affinity (Ki ≈10⁻¹⁴ M) and is active across pH 5.5–8.5. Include DTT or β-mercaptoethanol (1–5 mM) to maintain the inhibitor in its reduced, active form.
Recommended usage is 1–4 U/µL of reaction volume. For co-immunoprecipitation of RNA-binding proteins or sensitive single-cell applications, 4–8 U/µL is advised to ensure complete RNase suppression.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
References
- Sadikan MZ; Abdul Nasir NA; Bakar NS et al.. Tocotrienol-rich fraction reduces retinal inflammation and angiogenesis in rats with streptozotocin-induced diabetes. BMC complementary medicine and therapies. 2023;23:179. DOI
