| Field | Specification |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase RNF103; hKF-1; KF-1; RING finger protein 103; RN103_HUMAN; RNF 103; Rnf103; ZFP 103; Zfp-103; Zinc Finger Protein 103; Zinc finger protein 103 homolog; Zinc finger protein expressed in cerebellum; Zinc finger protein homologous to Zfp103 in mouse |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human E3 ubiquitin-protein ligase RNF103 protein (432-685AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-RNF103 antibody raised in Rabbit, with confirmed utility in ELISA, IHC, IF. Designed to detect RNF103 in Human, supporting researchers in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Human E3 ubiquitin-protein ligase RNF103 protein (432-685AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
- Host (Rabbit): Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
- Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
- Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A/G compatibility.
- Purification (Protein G purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
Biological background
RNF103 (also known as E3 ubiquitin-protein ligase RNF103, hKF-1, KF-1, RING finger protein 103, RN103_HUMAN, RNF 103, Rnf103, ZFP 103, Zfp-103, Zinc Finger Protein 103, Zinc finger protein 103 homolog, Zinc finger protein expressed in cerebellum, Zinc finger protein homologous to Zfp103 in mouse) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of RNF103 biology relevant to others.
Common research applications
- ELISA: Sandwich or indirect ELISA quantifies soluble/secreted target in biological fluids or cell culture supernatants.
- IHC: IHC visualizes target expression in FFPE or frozen sections; optimize antigen retrieval and secondary detection per tissue type.
- IF: IF localizes target at subcellular resolution in fixed cells/sections; fixation and permeabilization conditions affect epitope accessibility.
Notes for experimental interpretation
- Isotype controls: Include IgG from Rabbit at equivalent concentration to assess non-specific background.
- Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
- Species reactivity: Confirmed for Human; extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
RNF103 is a protein target in Human biology. This polyclonal antibody raised in Rabbit detects RNF103 and is validated for ELISA, IHC, IF applications.
This antibody is reported reactive against Human. Immunogen derived from Human. Cross-reactivity with unlisted species should not be assumed — contact us or consult the datasheet for homology data.
Supplier reports performance in ELISA, IHC, IF. Dilution optimization, blocking, and appropriate controls are required per format. Contact BioHippo for recommended working concentrations.
This non-conjugated (IgG) antibody requires a secondary anti-rabbit IgG conjugated to HRP, AP, fluorophore, or biotin. Match the secondary to the host (Rabbit) and isotype (IgG).
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — lysate/cell line expressing RNF103; (3) Negative control — siRNA KD or KO sample to confirm specificity; (4) Dilution linearity — verify proportional signal decrease for quantitative applications.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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