| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | Up to 100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: 5-[(4,5-dimethoxy-2-propan-2-ylphenyl)methyl]pyrimidine-2,4-diamine.
- CAS number: 1026582-88-6
- Molecular formula: C16H22N4O2.
- Purity: >97% (HPLC)
- Concentration: 50 nM - 50 µM.
- Form: Lyophilized powder.
- Solubility: Up to 100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
- Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: P2X3 receptors
- Source: Synthetic
- Activity: RO-3 is a potent and selective antagonist of the P2X3 and P2X2/3 receptors. It inhibits human homomeric P2X3 and heteromeric P2X2/3 receptors with pIC50 values of 7.0 and 5.9, respectively, whilst showing selectivity over other homomeric P2X family receptors with pIC50 of < 51.
Scientific background
RO-3 is a potent inhibitor of human homomultimeric P2X3 receptor and heteromultimeric P2X2/3 receptor and demonstrates pIC50 value of 7.0 and pIC50=5.9 respectively1.The compound shows to have to high metabolic stability in rat and human hepatocytes and liver microsomes. It's highly permeable, orally bioavailable, and has a good in vivo plasma half-life in rats. RO-3 is also widely distributed to tissues after administration, with low plasma protein binding and good CNS penetration1.The P2X receptors are a family of ion channels that are gated by ATP. P2X receptors are widely localized in cell types of almost every origin, including neuronal, muscular, epithelial, and immune and have been shown to play a pivotal role in models of various pain conditions2.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
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- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
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