| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
RORgammat is an immune-cell-specific isoform of the retinoic acid receptor-related orphan receptor gamma (RORC), a ligand-responsive nuclear receptor. It serves as the master transcription factor for the differentiation of T helper 17 (Th17) cells and is also expressed in group 3 innate lymphoid cells and other lymphocyte populations. By directly activating signature Th17 genes, including the gene encoding interleukin-17, RORgammat shapes mucosal immunity and host defense against extracellular pathogens. Dysregulated RORgammat activity contributes to chronic inflammation and autoimmune disorders such as psoriasis, inflammatory bowel disease, and multiple sclerosis. The receptor is therefore an important target for immunology research and for the development of inhibitors aimed at modulating Th17-driven inflammation.
Product Description & Applications
The RORgammat Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of RORgammat activity in mammalian cells. The construct contains tandem repeats of response elements designed to preferentially detect RORgammat over other ROR isoforms, placed upstream of a minimal promoter driving the reporter gene, with an optimized upstream enhancer that maximizes signal-to-noise. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines. Stable lentiviral integration provides consistent reporter expression suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is well suited to studying RORgammat activity and Th17 biology in primary and difficult-to-transfect cells.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the RORgt transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
