| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
The ROR response element (RORE) is the DNA sequence bound by the retinoic acid receptor-related orphan receptors, a family of nuclear receptors comprising RORα, RORβ, and RORγ. These transcription factors regulate genes involved in metabolism, circadian rhythm, and immune function. The RORγ isoform RORγt is selectively expressed in immune cells and acts as a direct activator of Th17 signature genes, making it a master regulator of Th17 cell differentiation. Because Th17 cells contribute to host defense and to autoimmune and inflammatory disease, ROR transcription factors, and RORγt in particular, are important targets in immunology research and therapeutic development.
Product Description & Applications
The RORE Reporter Lentivirus is a lentiviral reporter system for measuring the transcriptional activity of ROR family transcription factors in mammalian cells. Tandem repeats of the consensus ROR response element drive expression of a fluorescent (GFP, RFP) or luminescent (firefly or Renilla luciferase) reporter, providing a sensitive readout of RORα, RORβ, and RORγ activity, including the immune-specific RORγt isoform. The product is used to study ROR-mediated transcription, Th17 cell biology, and modulators of ROR activity. Antibiotic selection markers (puromycin or blasticidin) support establishment of stable reporter cell lines. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the ROR transcription factors transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
