RPMI 1788 cell

SKU:BHC11100606
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Overview
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RPMI 1788 cell is a B lymphoblast cell line derived from Caucasian (Male). It is commonly used as an in vitro model for 2 research. Growth characteristics: Adherent, Round cells. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Morphology Round cells
Growth Properties Adherent
Tissue Peripheral blood
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Catalog no. Size
300318 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 300318
Species Human
The RPMI 1788 cell line was derived from the peripheral blood of an apparently normal patient. The cells are EBNA positive.

SKU:BHC11100606

  • Antigen expression: HLA A2, Aw33, B7, B14
  • Isoenzymes: G6PD, B
  • Viruses: EBNA-pos
  • Reverse transcriptase: Negative
  • Products: IgM (lambda light chain), lymphotoxin (LT) also known as tumor necrosis factor beta (TNF-beta, TNF beta)
  • Karyotype: Human male, hypodiploid, stable
  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% FBS
  • subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth.
  • seedingDensity: 1 x 105 cells/mL
  • postThawRecovery: Low viability after thawing. Good recovery after 8 days
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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