| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RPS3A recombinant protein (Position: K18-R146) was used as the immunogen for the RPS3A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RPS3A Antibody / 40S ribosomal protein S3a is a anti-RPS3A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nucleolar.
Key elements and design rationale
- Target: RPS3A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
RPS3A is encoded by the RPS3A gene on human chromosome 4q31.3. The protein is approximately 30 kilodaltons and associates with 18S rRNA in the 40S ribosomal subunit. It stabilizes rRNA folding and coordinates interactions between ribosomal proteins during translation initiation. RPS3A expression is ubiquitous but particularly elevated in rapidly dividing cells, reflecting its link to growth and biosynthetic capacity.
The RPS3A antibody detects a 30 kilodalton protein by western blot and shows cytoplasmic and nucleolar staining under immunofluorescence. RPS3A interacts with translation initiation factors and regulates mRNA selection and ribosome recruitment. It also influences mitochondrial protein synthesis and cellular energy metabolism.
Outside the ribosome, RPS3A acts as a signaling modulator, interacting with viral proteins, apoptotic regulators, and stress response factors. Overexpression of RPS3A confers resistance to apoptosis and contributes to oncogenic transformation, while knockdown induces cell cycle arrest and autophagy. Elevated RPS3A expression has been detected in breast, liver, and lung cancers, correlating with poor prognosis and aggressive tumor behavior.
Due to its dual role in translation and signaling, RPS3A serves as a valuable biomarker for cell proliferation and stress adaptation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
