RPS6KA3 Antibody, FITC conjugated

Overview

This polyclonal anti-RPS6KA3 antibody raised in Rabbit, conjugated with FITC. Designed to detect RPS6KA3 in Human for signal transduction research.

Key elements and design rationale

• Immunogen: Recombinant Human Ribosomal protein S6 kinase alpha-3 protein (214-431AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
• FITC: Direct fluorescence (Ex490/Em525); protect from light; use antifade.

Biological background

RPS6KA3 (also: 90 kDa ribosomal protein S6 kinase 3, CLS, HU 3, HU2, HU3, Insulin stimulated protein kinase 1, Insulin-stimulated protein kinase 1, ISPK-1, ISPK1, KS6A3_HUMAN, MAP kinase activated protein kinase 1b, MAP kinase-activated protein kinase 1b, MAPK activated protein kinase 1b, MAPK-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK 1b, MAPKAPK-1b, MAPKAPK1B, Mental retardation, X linked 19, MRX19, OTTHUMP00000023036, p90 RSK2, p90 RSK3 , p90-RSK 3, p90RSK3, pp90RSK2, Ribosomal protein S6 kinase 90kDa polypeptide 3, Ribosomal protein S6 kinase alpha 3, Ribosomal protein S6 kinase alpha-3, Ribosomal protein s6 kinase ii alpha 2 , Ribosomal S6 kinase 2, Rps6ka3, RSK, RSK-2, RSK2, S6 kinase 2, S6K alpha3, S6K-alpha-3) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current RPS6KA3 annotation relevant to signal transduction.

Common research applications

• Refer to product documentation for confirmed application data.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.