RRE Reporter Lentivirus

Background

The RAS-ERK pathway is a central signaling cascade in which growth factor activation of RAS GTPases drives the RAF-MEK-ERK kinase module, ultimately altering gene transcription that governs cell proliferation, differentiation, and survival. Activated ERK regulates transcription factors that bind the Ras-responsive element (RRE), including ETS family members such as ERG, ETV1, ETV4, and ETV5, as well as RREB-1. These factors mediate the gene expression changes associated with oncogenic transformation. Aberrant RAS-ERK signaling, whether through RAS mutation or ETS gene rearrangement, is a frequent driver of human cancers, making RRE-dependent transcription an informative readout of pathway activity and a target for inhibitor discovery.

Product Description & Applications

The RRE Reporter Lentivirus is a transcription-factor reporter system designed to detect transcription downstream of RAS-ERK signaling. The construct contains tandem repeats of the Ras-responsive element coupled to a minimal promoter, driving a fluorescent or luminescent reporter chosen from options including GFP, RFP, mCherry, BFP2, firefly luciferase, Gaussia luciferase, and dual configurations. A constitutively expressed selection marker supports stable polyclonal cell line generation, with readout by microscopy, flow cytometry, or luminometry.

The system is suited to monitoring RAS-ERK pathway activation, screening pathway inhibitors, and studying oncogenic signaling driven by ETS family transcription factors and RREB-1. Particles are purified by PEG precipitation and sucrose gradient centrifugation for effective transduction of primary and thawed cells.

About This Product

This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the RAS-ERK Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.

Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.