| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Storage |
Description
RT-LAMP Dye Assay Kit (UDG plus) contains fluorescent dye, Hieff™ Bst Plus DNA Polymerase, heat-resistant Hifair™ Ⅲ Reverse Transcriptase, MRI, and UDGas applied to LAMP reaction with RNA as template. This product is with high sensitivity and high specificity.
Features
- High Sensitivity：50 copies/T, 25 μL system;
- High stability: stable at 37°C for 5 days with good stability, and can withstand repeated freezing and thawing more than 10 times;
- Specificity: 48-well NTC has no peaks at all, and the specificity is strong;
Applications
- Rapid detection of pathogenic bacteria
- Rapid detection of pet pathogens
- On-site detection of animal husbandry
Specifications
| Detection method | Fluorescent dye method |
| PCR method | RT-LAMP |
| Polymerase | Hieff™ Bst Plus DNA Polymerase |
| Type of sample | RNA |
| LOD | 50 copies/T |
| Specificity | 100% |
Components
| Components No. | Name | 13762ES60 (100 T) | 13762ES80 (1000 T) |
| 13762-A | Bst Buffer | 600 µL | 6 mL |
| 13762-B | Bst Enzyme Mix（UDG plus） | 100 µL | 1 mL |
Storage
Part 1: 13762-A should be protected from light and can be stored at -20°C for 6 months.
Part 2: 13762-B can be stored at -20°C for 6 months.
Figures
- High Sensitivity
Figure 1: Sensitivity Test
Red: 50 copies/T, response less than 15 minutes
Figure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%.
- Stability
Figure 2.Stability testing at the same temperature
Red: 37℃ 0 day Blue: 37℃ 1 day Green: 37℃ 3 days Purple: 37℃ 5 days
0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.
Figure 3. -20℃ repeated freeze-thaw stability test
Red: Repeat freeze-thaw 0 times
Blue: Repeat freeze-thaw 5 times
Green: Repeat freeze-thaw 10 times
- Specificity
Figure 4. 48-well NTC-specific assay
48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better.
RT-LAMP and LAMP reactions in this kit proceed isothermally at 60–65°C (optimized temperature specified in the datasheet). A standard heat block, water bath, or isothermal dry-bath incubator is sufficient — no thermocycler is required. This makes the kit well-suited to resource-limited or point-of-care settings where conventional PCR instrumentation is unavailable.
The Hotstart Bst Plus enzyme used in this kit incorporates an antibody-mediated cold-block that prevents non-specific amplification and false-positive results at ambient temperature. Activity is released upon incubation at ≥60°C, improving assay specificity and enabling pre-reaction setup at room temperature without risk of non-specific product formation.
Detection method depends on the kit format: Dye Assay kits use intercalating fluorescent dyes (e.g., SYBR Green, calcein, or YSFluor) for visual or instrument-based detection; Fluorescent Assay kits use real-time fluorescence monitoring; Probe Assay kits use sequence-specific probes for enhanced specificity. Refer to the product title and datasheet for the exact detection format in the kit you ordered.
This kit is validated for purified RNA or DNA templates. Some formats support direct amplification from swabs, saliva, or crude lysates without prior nucleic acid extraction — check the specific product datasheet for the validated sample input types, inhibitor tolerances, and any pre-treatment requirements.
LAMP amplification typically completes within 20–60 minutes at the isothermal incubation temperature, compared to 1.5–3 hours for conventional RT-PCR. The faster time-to-result makes this kit especially useful for high-throughput screening workflows and time-sensitive applications such as outbreak response and quarantine protocols.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
