| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Storage |
Description
The RT-LAMP Fluorescent Assay Kit (Bst Plus) is a kit capable of isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant HifairTM III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve highly efficient, rapid, and highly specific detection reactions, and supports room-temperature reaction system preparation.
Features
- Broad Compatibility: Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products.
- High Specificity: Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors.
- Excellent Sensitivity: Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction.
- Robust Stability: Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup.
Components
|
Components No. |
Name |
16729ES60 |
16729ES80 |
|
16729-A |
4×LAMP Buffer |
600 µL |
6 mL |
|
16729-B |
Bst Plus DNA Polymerase |
100 µL |
1 mL |
|
16729-C |
RT Enzyme mix |
50 µL |
500 µL |
|
16729-D |
25×Fluorescent Dye |
100 µL |
1 mL |
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.Component D must be stored away from light.
Application
Isothermal Amplification;Rapid detection of pathogenic bacteria.
Figures
1. Workflow
Figure 1. RT-LAMP Workflow.
2. Broad Compatibility: DNA Pathogen Detection&RNA Pathogen Detection
Figure 2. Broad Pathogen Detection Compatibility.
(A) DNA pathogen detection: Reliable amplification observed across multiple DNA virus targets.
(B) RNA pathogen detection: Effective detection of RNA viruses with high sensitivity and specificity.
3. High Specificity
Figure 3. High Specificity of RT-LAMP Assay (Cat#16729) Compared to Supplier A*
Specificity was evaluated using both DNA and RNA templates, including positive targets and non-target (negative) controls. The RT-LAMP assay (Cat#16729) showed no false positives and clear separation between positive and negative samples, outperforming Supplier A* by avoiding non-specific amplification.
4. Strong stability
Figure 4. Stability Evaluation of the RT-LAMP System.
(A) Freeze-thaw stability: Performance remains consistent after 20 freeze-thaw cycles.
(B) Thermal accelerated stability: Maintains amplification efficiency after 7 days of storage at 37°C.
Documents:
Safety Data Sheet
Manuals
16729_Manual_Ver.EN20250708.pdf
RT-LAMP and LAMP reactions in this kit proceed isothermally at 60–65°C (optimized temperature specified in the datasheet). A standard heat block, water bath, or isothermal dry-bath incubator is sufficient — no thermocycler is required. This makes the kit well-suited to resource-limited or point-of-care settings where conventional PCR instrumentation is unavailable.
The Hotstart Bst Plus enzyme used in this kit incorporates an antibody-mediated cold-block that prevents non-specific amplification and false-positive results at ambient temperature. Activity is released upon incubation at ≥60°C, improving assay specificity and enabling pre-reaction setup at room temperature without risk of non-specific product formation.
Detection method depends on the kit format: Dye Assay kits use intercalating fluorescent dyes (e.g., SYBR Green, calcein, or YSFluor) for visual or instrument-based detection; Fluorescent Assay kits use real-time fluorescence monitoring; Probe Assay kits use sequence-specific probes for enhanced specificity. Refer to the product title and datasheet for the exact detection format in the kit you ordered.
This kit is validated for purified RNA or DNA templates. Some formats support direct amplification from swabs, saliva, or crude lysates without prior nucleic acid extraction — check the specific product datasheet for the validated sample input types, inhibitor tolerances, and any pre-treatment requirements.
LAMP amplification typically completes within 20–60 minutes at the isothermal incubation temperature, compared to 1.5–3 hours for conventional RT-PCR. The faster time-to-result makes this kit especially useful for high-throughput screening workflows and time-sensitive applications such as outbreak response and quarantine protocols.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
