| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Storage |
Description
The RT-LAMP Probe Assay Kit is a reagent designed for LAMP amplification using DNA and RNA templates. It includes the next-generation Bst Plus HSS DNA Polymerase, heat-resistant Hifair™ III Reverse Transcriptase, MRI, and an optimized buffering system, allowing for rapid and efficient LAMP/RT-LAMP probe-based detection. The Bst Plus HSS DNA Polymerase employs an aptamer-based hot start mechanism that suppresses enzymatic activity at ambient temperatures, enhancing specificity and sensitivity while enabling stable reaction assembly at room temperature.
Features
- Hotstart Bst DNA Pol
- High Sensitivity
Components
|
Components No. |
Name |
16903ES60 |
|
16903-A |
4×RT-LAMP Buffer (HSS) |
600 µL |
|
16903-B |
RT-LAMP Enzyme mix (HSS) |
100 µL |
Specifications
|
Detection method |
RT-LAMP Fluorescent probe method |
|
Enzyme mix |
Bst Plus HSS DNA Polymerase; Hifair™ III Reverse Transcriptase |
|
Type of sample |
RNA |
|
Application |
Isothermal amplification reaction |
Shipping and Storage
The product should be stored at -15℃ ~ -25℃ for 1 year. Please avoid light.
Operating Instructions
|
Components |
Volume (μL) |
Final Concentration |
|
4×RT-LAMP Buffer(HSS) |
6 |
1 × |
|
RT-LAMP Enzyme mix(HSS) |
1 |
- |
|
Primer mix* |
2.5 |
- |
|
Template |
1-10 |
- |
|
ddH2O |
up to 25 |
- |
Notes:
Recommended primer set at 16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LoopF/LoopB; the probe can be added at the optimal amount confirmed by the design scheme, and the ratio of other optimal primer sets can be tested.
Reaction conditions
Incubate at a constant temperature of 63-65°C for 30-60 minutes, followed by deactivation at 85°C for 5 minutes.
Notes
For your safety and health,please wear lab coats and disposable gloves for operation.
RT-LAMP and LAMP reactions in this kit proceed isothermally at 60–65°C (optimized temperature specified in the datasheet). A standard heat block, water bath, or isothermal dry-bath incubator is sufficient — no thermocycler is required. This makes the kit well-suited to resource-limited or point-of-care settings where conventional PCR instrumentation is unavailable.
The Hotstart Bst Plus enzyme used in this kit incorporates an antibody-mediated cold-block that prevents non-specific amplification and false-positive results at ambient temperature. Activity is released upon incubation at ≥60°C, improving assay specificity and enabling pre-reaction setup at room temperature without risk of non-specific product formation.
Detection method depends on the kit format: Dye Assay kits use intercalating fluorescent dyes (e.g., SYBR Green, calcein, or YSFluor) for visual or instrument-based detection; Fluorescent Assay kits use real-time fluorescence monitoring; Probe Assay kits use sequence-specific probes for enhanced specificity. Refer to the product title and datasheet for the exact detection format in the kit you ordered.
This kit is validated for purified RNA or DNA templates. Some formats support direct amplification from swabs, saliva, or crude lysates without prior nucleic acid extraction — check the specific product datasheet for the validated sample input types, inhibitor tolerances, and any pre-treatment requirements.
LAMP amplification typically completes within 20–60 minutes at the isothermal incubation temperature, compared to 1.5–3 hours for conventional RT-PCR. The faster time-to-result makes this kit especially useful for high-throughput screening workflows and time-sensitive applications such as outbreak response and quarantine protocols.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
