RUNX3/RUNX2/RUNX1 Recombinant Monoclonal Antibody

Overview

This is a monoclonal anti-RUNX3/RUNX2/RUNX1 antibody raised in Rabbit, with confirmed utility in ELISA, FC. It is designed to detect RUNX3/RUNX2/RUNX1 protein in Human and supports researchers working in others contexts.

Key elements and design rationale

• Immunogen: A synthesized peptide derived from human RUNX3/RUNX2/RUNX1 — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
• Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
• Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
• Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
• Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.

Biological background

RUNX3/RUNX2/RUNX1 (also referred to as Acute myeloid leukemia 2 protein, Acute myeloid leukemia gene 2, AML 2, AML2, CBF alpha 3, CBF-alpha-3, CBFA 3, CBFA3, Core binding factor alpha 3 subunit, core binding factor, Core binding factor runt domain alpha subunit 3, Core binding factor subunit alpha 3, core-binding factor, Core-binding factor subunit alpha-3, Oncogene AML 2, Oncogene AML-2, PEA2 alpha C, PEA2-alpha C, PEBP2 alpha C, PEBP2-alpha C, Pebp2a3, PEBP2aC, Polyomavirus enhancer binding protein 2 alpha C subunit, Polyomavirus enhancer-binding protein 2 alpha C subunit, runt domain alpha subunit 3, runt related transcription factor 3, Runt-related transcription factor 3, RUNX 3, Runx3, RUNX3_HUMAN, SL3 3 enhancer factor 1 alpha C subunit, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core binding factor alpha C subunit, SL3/AKV core-binding factor alpha C subunit, Transcription factor AML2Acute myeloid leukemia 1, Acute myeloid leukemia 1 protein, alpha subunit core binding factor, AML 1, AML1, AML1 EVI 1, AML1 EVI 1 fusion protein, Aml1 oncogene, AMLCR 1, AMLCR1, CBF alpha 2, CBF-alpha-2, CBFA 2, CBFA2, Core binding factor alpha 2 subunit, Core binding factor runt domain alpha subunit 2, Core-binding factor subunit alpha-2, EVI 1, EVI1, HGNC, Oncogene AML 1, Oncogene AML-1, OTTHUMP00000108696, OTTHUMP00000108697, OTTHUMP00000108699, OTTHUMP00000108700, OTTHUMP00000108702, PEA2 alpha B, PEA2-alpha B, PEBP2 alpha B, PEBP2-alpha B, PEBP2A2, PEBP2aB, Polyomavirus enhancer binding protein 2 alpha B subunit, Polyomavirus enhancer-binding protein 2 alpha B subunit, Run1, Runt related transcription factor 1, Runt-related transcription factor 1, RUNX 1, Runx1, RUNX1_HUMAN, SL3 3 enhancer factor 1 alpha B subunit, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core binding factor alpha B subunit, SL3/AKV core-binding factor alpha B subunitAcute myeloid leukemia 3 protein, Alpha subunit 1, AML3, CBF alpha 1, CBF-alpha-1, CBFA1, CCD, CCD1, Cleidocranial dysplasia 1, Core binding factor, Core binding factor runt domain alpha subunit 1, Core binding factor subunit alpha 1, Core-binding factor subunit alpha-1, MGC120022, MGC120023, Oncogene AML 3, Oncogene AML-3, OSF 2, OSF-2, OSF2, Osteoblast specific transcription factor 2, Osteoblast-specific transcription factor 2, OTTHUMP00000016533, PEA2 alpha A, PEA2-alpha A, PEA2aA, PEBP2 alpha A, PEBP2-alpha A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, Polyomavirus enhancer binding protein 2 alpha A subunit, Polyomavirus enhancer-binding protein 2 alpha A subunit, Runt domain, Runt related transcription factor 2, Runt-related transcription factor 2, RUNX2, RUNX2_HUMAN, SL3 3 enhancer factor 1 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core binding factor alpha A subunit, SL3/AKV core-binding factor alpha A subunit) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of RUNX3/RUNX2/RUNX1 biology in others.

Common research applications

• ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
• FC: Applicable for FC-based detection; refer to product documentation for optimized conditions.

Notes for experimental interpretation

• Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
• Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
• Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
• Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.

This product is a recombinant monoclonal antibody expressed in HEK293F cells.

• Datasheet (12942711)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.