S. Aureus Purified CP8

What it means: This CP8 is a natively purified capsular polysaccharide isolated from two S. aureus strains — Reynolds and O13 — providing broad structural representation of the serotype 8 polysaccharide.

Why it matters:

• Dual-strain sourcing from Reynolds and O13 ensures the CP8 preparation captures the structural heterogeneity seen across clinical serotype 8 isolates.
• CP8 identity is confirmed by monoclonal antibody 5A6, providing reliable lot-to-lot quality control and serotype specificity assurance.

How to interpret here: This S. aureus CP8 is purified from the established Reynolds and O13 reference strains, making it compatible with published OPA assay and vaccine immunogenicity protocols that specify these source strains.

What it means: Form describes the physical state in which the product is supplied. This CP8 is lyophilized (freeze-dried) in sterile water.

Why it matters:

• Lyophilized polysaccharides offer superior long-term stability at -20°C compared to liquid formulations, preventing structural degradation over extended storage.
• Reconstitute in sterile water to your target working concentration before use in ELISA inhibition, Western Blot, or OPA assays.
• Single-use aliquots after reconstitution prevent repeat freeze-thaw damage.

How to interpret here: This lyophilized S. aureus CP8 must be reconstituted in sterile water before use. Store at -20°C before and after reconstitution.

What it means: The target pathogen is Staphylococcus aureus — a leading Gram-positive pathogen. This product is the serotype 8 capsular polysaccharide (CP8), the second major capsular serotype alongside CP5.

Why it matters:

• CP5 and CP8 together are expressed by ~80% of clinical S. aureus bloodstream isolates — CP8 antibodies do not cross-react with CP5, making serotype-correct antigen selection essential for accurate serology.
• CP8 is a key antigen in S. aureus vaccine development and a reference standard for serotype 8-specific OPA assays.

How to interpret here: Use this S. aureus CP8 for serotype 8-specific immunological assays. For serotype 5 coverage, pair with S. aureus Purified CP5 (BHP20900071).

What it means: Each unit supplies 100 µg of lyophilized S. aureus CP8, requiring reconstitution in sterile water to the desired working concentration.

Why it matters:

• The correct reconstitution volume determines working concentration — critical for reproducible ELISA inhibition and OPA assay outcomes.
• Unlike liquid reagents with a defined mg/mL value, lyophilized products require user-defined reconstitution; plan this step based on your assay's antigen coating or inhibition concentration requirements.

How to interpret here: Reconstitute this 100 µg lyophilized S. aureus CP8 in sterile water. Determine the appropriate working concentration empirically for your assay format.

What it means: Storage gives the manufacturer-recommended condition: -20°C, per the IBT Bioservices datasheet.

Why it matters:

• -20°C storage maintains lyophilized polysaccharide integrity and serological reactivity for extended periods — polysaccharides are susceptible to hydrolytic degradation at higher temperatures.
• After reconstitution, aliquot and return to -20°C immediately; avoid prolonged storage at 4°C post-reconstitution.

How to interpret here: Store this S. aureus CP8 at -20°C in lyophilized form and after reconstitution. Each reconstituted aliquot should be used in a single experiment session.