| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Salt Active UltraNuclease is a nonspecific endonuclease and has optimum activity at high salt concentration. Salt can play an important role in minimizing aggregation of protein or virus, which would be helpful for dissociation of DNA & RNA from proteins or other cellular components. In high salt conditions, enzymes are more likely to access the released DNA & RNA and degrade them. Nuclease which has high activity at high salt would be more effective to improve purification processes. The Salt Active UltraNuclease can be used to reduce the viscosity of cell supernatant and cell lysate, increase purification efficiency in high salt condition.
The enzyme can also reduce host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing.
Features
High Activity in High-Salt Conditions: Maintains optimal enzyme activity even at 500 mM salt concentration.
Enhanced Viral Yield: High-salt tolerance reduces AAV particle aggregation, improving virus recovery.
Reliable Product Quality: GMP-grade, animal-free, and antibiotic-free.
Regulatory Support: DMF filing completed (MF037815).
High Purity: ≥98% purity ensures consistent performance.
Low Endotoxin: Suitable for sensitive applications.
Compatible with ELISA Kits: Fully supported for downstream ELISA-based analysis.
Specification
|
Expression Host |
Recombinant, expressed in Escherichia coli |
|
Molecular Weight |
24.7 kDa |
|
Isoelectric point |
9.61 |
|
Purity |
≥ 99% |
|
Enzyme activity |
250-300 U/μL |
|
Specific activity |
≥3×105 U/mg |
|
Protein purity (SEC-HPLC) |
≥99% |
|
Bacterial endotoxins(gel-clot） |
<0.25 EU/1000U |
|
Host Protein Residue(ELISA) |
<10 ppm(μg/mL) |
|
Optimum Temperature |
37°C (operating range 0-42°C) |
|
Cofactor |
1-10 mM Mg2+ |
|
Storage Buffer |
25 mM Tris-HCl，5 mM MgCl2，500 mM NaCl，50% glycerol |
|
Unit Definition |
The definition of one activity unit (U) is the amount of enzyme that causes a △A260=1.0 in 30 minutes at 37℃ in the excess of substrate at the certain condition. |
Figures
1. Robust Activity in High-Salt Environments
Figure 1. Enzyme activity under high-salt conditions.
(A). UltraNuclease maintains robust activity in high-salt environments, showing maximum activity at 500 mM salt.
(B). Compared with Supplier M*, Yeasen UltraNuclease (Cat#20159ES) demonstrated lower HCD residuals, particularly under 400 mM NaCl with 1% Tween.
2. Efficient Nucleic Acid Removal: AAV Production Case
|
Kit |
Condition (100U/mL, 37℃, 1 h) |
HCD residuals（ng/mL) |
|
Supplier M* |
400mM NaCl |
1.42 |
|
Supplier M* |
400mM NaCl, 1% Tween |
0.68 |
|
Yeasen 20159ES |
400mM NaCl |
0.87 |
|
Yeasen 20159ES |
400mM NaCl, 1% Tween |
0.07 |
Compared with Supplier M*, Yeasen UltraNuclease (Cat#20159ES) demonstrated lower HCD residuals, particularly under 400 mM NaCl with 1% Tween.
3. Recommended operating conditions
|
Condition |
Optimal |
Effective |
|
Mg2+/Mn2+ |
10~50 mM |
1~100 mM |
|
pH |
8 |
6.5~10 |
|
Temperature |
37℃ |
4~42℃ |
|
Na+/K+ |
500 mM |
300~1200 mM |
4. Recommended Usage
|
Condition |
Suggested usage |
|
Protein |
100 U/mL |
|
Cell lysate |
500 U/mL |
|
Cell extract |
1000 U/mL |
|
Viscosity reduction |
50 U/mL |
5. Strong stability
Figure 2. Stability of UltraNuclease.
(A) Enzyme activity remained unchanged after 4 weeks of storage at –20 °C and 4 °C, and after 2 weeks at 37 °C.
(B) Enzyme activity was unaffected after 10 freeze–thaw cycles between –80 °C and –25 °C.
Shipping and Storage
The product should be stored at -25 ~ -15℃ for two years. If the product is opened and has been stored at 4℃ for more than one week, we recommend filtering the product to prevent microbial contamination.
Glycerol Content: Contains Glycerol
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in Nucleic Acid Purification applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U) is defined as the amount of enzyme that degrades 1 µg of DNA or RNA substrate to acid-soluble form in 30 min at 37°C under defined buffer conditions.
This enzyme is produced as Recombinant (E. coli) and is available in GMP grade for regulated applications. This enzyme is manufactured in an ISO 13485-certified UCF.ME™ facility and meets GMP-grade quality standards. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Nuclease activity typically requires divalent metal ion cofactors (Mg²⁺ or Mn²⁺ at 1–5 mM). Activity is inhibited by chelating agents such as EDTA (≥1 mM), high salt concentrations, and reducing agents such as DTT at elevated concentrations. Heat inactivation (65–75°C for 15 min) is effective for most nucleases, allowing removal of enzyme activity after digestion without column purification.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
