| Field | Specification |
|---|---|
| Mfr No | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant SARS-COV-2 Nucleocapsid (B.1.1.529/Omicron) Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser2-Ala419 (P13L, E31del, R32del, S33del, R203K, G204R) ) of sars-cov-2 N (B.1.1.529/Omicron) (Accession #QHD43423.2) fused with a 6×His tag at the C-terminus. |
| Product Type | |
| Purity | |
| Storage |
Scientific Background
The SARS-CoV-2 nucleocapsid (N) protein encapsidates the viral RNA genome into helical ribonucleoprotein (RNP) complexes essential for replication and assembly. As one of the most abundantly expressed and immunogenic SARS-CoV-2 proteins, the N protein is a primary target for serology assays, COVID-19 diagnostics, and pan-coronavirus vaccine development. The Omicron (B.1.1.529) variant N protein carries R203K and G204R mutations that alter RNA binding and phase-separation behaviour.
Product Description
E. coli ExpressionSARS-COV-2 Nucleocapsid (B.1.1.529/Omicron) is supplied as a recombinant protein with C-His. The protein spans Ser2-Ala419. It is produced using a validated recombinant expression system and supplied in the format indicated (lyophilized or liquid), suitable for use in biochemical binding assays, ELISA standards, antibody validation, and related research applications.
Protein Specifications
| Expression System | E. coli |
|---|---|
| Expression Region | Ser2-Ala419 |
| Purity | > 95% by SDS-PAGE. |
| Formulation | Supplied as a 0.22 Mu m filtered solution in 20mM Tris, 500mM NaCl, 0.1mM EDTA, 10% Glycerol, pH 8.0. |
| Storage | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Safety & Handling
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
This protein was produced in E. coli cells expression system. The expression system determines the post-translational modification profile, folding characteristics, and suitability for specific downstream applications.
Reconstitution instructions are provided on the Certificate of Analysis. Centrifuge the lyophilized vial briefly before opening, then add the recommended volume of sterile water or buffer and allow to dissolve at 4°C for 30 minutes without vortexing. Aliquot into single-use working portions in 0.1% BSA carrier protein to minimise freeze-thaw losses.
This protein meets > 95% by SDS-PAGE purity as assessed by SDS-PAGE.
This protein is suitable for use in biochemical binding assays, ELISA standard curves, antibody validation, SPR/BLI interaction studies, and structural research. Bioactivity data, where available, is provided on the Certificate of Analysis. Contact technical support for application-specific optimisation guidance.
Store at −20°C immediately upon receipt. Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.. Lyophilized proteins are generally stable for 12 months at −20°C. Reconstituted protein should be aliquoted and stored at −80°C.
BioHippo offers flexible sourcing and customisation options for qualified research institutions and commercial partners. The following add-ons may be available depending on supplier capabilities and order volume.
- Custom quantities: Bulk orders or non-standard pack sizes may be available for high-throughput screening or large-scale projects.
- Custom formulation: Alternative buffer compositions or lyophilisation formats may be accommodated on request.
- Extended QC documentation: Additional purity methods, endotoxin testing, or bioactivity assay data can be requested for GMP-adjacent applications.
Please contact BioHippo customer support to discuss your specific requirements. Customisation availability and lead times vary by product and supplier.
