SDHB Antibody, HRP conjugated

Overview

This is a polyclonal anti-SDHB antibody raised in Rabbit, conjugated with HRP, with confirmed utility in ELISA. It is designed to detect SDHB protein in Human and supports researchers working in signal transduction contexts.

Key elements and design rationale

• Immunogen: Recombinant Human Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial protein (29-280AA) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
• Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
• Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
• Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
• Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
• HRP conjugate: Direct detection with TMB/OPD or ECL substrates. Quench endogenous peroxidase before use in tissue assays.

Biological background

SDHB (also referred to as CWS2, DHSB_HUMAN, FLJ92337, Ip, Iron sulfur subunit, Iron sulfur subunit of complex II, Iron-sulfur subunit of complex II, mitochondrial, PGL 4, PGL4, SDH 1, SDH, SDH1, SDH2, SDH2, homolog of, SdhB, SDHIP, Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial, Succinate dehydrogenase [ubiquinone] iron sulfur subunit, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, Succinate Dehydrogenase 1 Iron Sulfur Subunit, Succinate Dehydrogenase 2, S. cerevisiae, homolog of, Succinate dehydrogenase complex iron sulfur subunit B, Succinate dehydrogenase complex subunit B iron sulfur, Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein, succinate dehydrogenase complex, subunit B, iron sulfur (Ip), Succinate dehydrogenase iron sulfur protein) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of SDHB biology in signal transduction.

Common research applications

• ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.

Notes for experimental interpretation

• Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
• Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
• Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
• Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12928256)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.