| Field | Specification |
|---|---|
| Applications | |
| Molecular weight | |
| Molecular formula | C53H83NO13 |
| SMILES | |
| Form | Oil |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Seco Rapamycin ethyl ester is an open-ring metabolite of a Rapamycin derivative. It has been reported not to affect mTOR function[1]. It is supplied as a light yellow to yellow oil (C53H83NO13, MW 942.23).
Physical & Chemical Properties
| Molecular Formula | C53H83NO13 |
|---|---|
| Molecular Weight | 942.23 g/mol |
| Appearance | Oil |
| Color | Light yellow to yellow |
| SMILES | C[C@H]1[C@@](C(C(N2[C@H](C(OCC)=O)CCCC2)=O)=O)(O)O[C@H](C[C@H](OC)/C(C)=C/C=C/C=C/[C@@H](C)C[C@@H](C)C([C@H](OC)[C@H](O)/C(C)=C/[C@@H](C)C(/C=C/[C@H](C)C[C@@H]3CC[C@@H](O)[C@H](OC)C3)=O)=O)CC1 |
| Signaling Pathway | Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: 100 mg/mL (106.13 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | -20°C, protect from light, stored under nitrogen. In solvent: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 100 mg/mL (106.13 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); protect from light, stored under nitrogen; avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | 5 mg/mL (5.31 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 5 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (50.0 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | 5 mg/mL (5.31 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 5 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (50.0 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Protocol 3
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 5 mg/mL (5.31 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 5 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (50.0 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
Seco Rapamycin disposition in human tissue homogenates and Caco-2 cell monolayers. Metabolism of Seco Rapamycin (D2) to dihydro Sirolimus (M2) is tested by incubating 20μM Seco Rapamycin with human liver, jejunal mucosal, and Caco-2 homogenates. All the homogenates generate M2 in an NADPH-dependent manner. Ketoconazole at a high concentration (100μM) has no effect on M2 formation in any of the homogenates examined. Metabolism of Seco Rapamycin to M2 in intact cells is tested by adding 20μM Seco Rapamycin to Caco-2 cell monolayers. With apical application, only a little Seco Rapamycin is found in the basolateral compartment and cellular fraction after 4 h, and little M2 is detected either. LY335979 has little effect on Seco Rapamycin distribution after an apical dose, although M2 becomes detectable in the apical compartment. By contrast, with basolateral application of Seco Rapamycin, Seco Rapamycin and M2 are both readily found in the apical compartment; LY335679 lowers Seco Rapamycin flux toward the apical compartment while raising M2 amounts in the apical and basolateral compartments[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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