Selective Tissue Staining Fluorescence Dye

What it means: Ex 485 nm / Em 535 nm — how the signal is read.

Why it matters:

• The readout decides which instrument you need.
• Wavelength settings must match the dye for the best signal.
• Knowing the readout helps you choose plate color and plate type.
• Running blanks with the same settings lets you subtract background.
• Stating instrument settings makes results easy to repeat.
• The signal window depends on how far sample readings are above the blank.
• Reading all plates with the same instrument settings keeps data comparable.
• Absorbance and fluorescence readouts need different plate types, so plan plates in advance.

For this product: Read the stain at Ex 485 nm / Em 535 nm.

Tip: Wash stained sections well; the dye binds tightly, which allows extensive washing for a low background.

What it means: 1000 x — the concentration of the supplied stock.

Why it matters:

• The stock concentration decides how far you dilute the product for each reaction.
• Knowing it lets you work out how many reactions one vial supports.
• Using the same final amount in every reaction keeps results comparable.
• A stated concentration makes dilution steps easy to record and repeat.
• It also helps you compare your conditions with published methods.
• Writing down the lot and stock concentration helps when you compare runs over time.
• Over-concentrated working solutions can raise background or waste material.

For this product: The dye is supplied as a 1000 x stock, 0.1 ml per vial.

Tip: Dilute only the amount needed for the day.

What it means: Frozen — how the product is shipped.

Why it matters:

• The shipping condition tells you what to expect when the package arrives.
• Unpacking promptly lets you check the contents against the order.
• Checking the package on arrival helps you spot transport problems early.
• Storage conditions can differ from shipping conditions, so confirm them before the package arrives.
• Recording the arrival date and condition helps if questions come up later.
• Arranging for someone to receive the package avoids delays when it arrives.
• The shipping condition is chosen to keep the product as described during transport.

For this product: Ships frozen; unpack it promptly on arrival. Please contact us to confirm storage conditions.

Tip: Open and check the package on arrival, and contact us right away if anything is damaged or missing.