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The SF126 cell line is a human glioblastoma cell line, widely utilized in research on brain tumors, particularly in studies exploring the molecular mechanisms of glioblastoma and its response to various treatments. Derived from a patient with glioblastoma multiforme, SF126 cells are known for their aggressive growth and invasive behavior, typical of glioblastomas, making them a crucial model for investigating therapeutic strategies and understanding tumor biology. One of the notable features of SF126 is its use in exploring both apoptosis (programmed cell death) and autophagy, as these processes are central to cancer cell survival and resistance to treatment.
SF126 has been extensively studied for its interactions with p53, a tumor suppressor gene frequently mutated in cancers. In SF126, researchers have investigated the effects of wild-type and mutant p53 on cell death mechanisms. It was found that p53 induces both apoptosis and autophagy, with autophagic cell death playing a significant role in p53-dependent cell death. This has implications for therapies targeting autophagic pathways, which may enhance the efficacy of treatments aimed at inducing tumor cell death. Additionally, studies have shown that manipulating autophagy can influence the overall tumor response to p53 activation, offering potential therapeutic angles for glioblastoma treatment.
Further research on SF126 has explored its binding properties with opioid peptides, such as β-endorphins, revealing specific binding sites for these molecules. This has provided insights into how glioblastoma cells might interact with endogenous hormones and signaling molecules in the brain, further underscoring the complexity of glioblastoma biology and potential novel therapeutic targets.
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- Tumorigenic: No (tested in athymic mice)
- Products: procollagen III, forms collagen fibers in vitro (interstitial collagen synthesis)
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use 50% basal medium + 40% FBS + 10% DMSO, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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