| Field | Specification |
|---|---|
| Mfr No | |
| Species |
Virus susceptibility: Baculoviruses, Autographa californica (MNPV), St. Louis encephalitis (SLE)
- cultureMedium: Spodopan (PAN Biotech)
- supplements: Supplement the medium with 2% FBS to enhance proliferation if needed
- dissociationReagent: Accutase
- subculturing: Detachment of cells via a cell scraper is recommended. Collect the medium with detached cells after scraping in a 15ml centrifuge tube. Add about 5ml of medium to the flask and rinse the flask several times to collect any remaining cells and combine them with the rest of the cells in the tube. Centrifuge for 3 min at 300xg, remove the supernatant, resuspend the cells in fresh, cold medium and dispense into new flasks.
- seedingDensity: 1 x 104 cells/cm2. Incubate between 26 to 30 degree Celsius in a non-humidified, ambient air-regulated incubator. Use cell culture flasks with filter caps or loosen caps to allow for oxygen exchange.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.